Genetic structure and geographical variation of Bithynia siamensis goniomphalos sensu lato (Gastropoda: Bithyniidae), the snail intermediate host of Opisthorchis viverrini sensu lato (Digenea: Opisthorchiidae) in the Lower Mekong Basin revealed by mitochondrial DNA sequences

被引：16

作者：

Tantrawatpan, Chairat ^{[1
]}

Saijuntha, Weerachai ^{[2
]}

Bunchom, Naruemon ^{[2
]}

Suksavate, Warong ^{[3
]}

Pilap, Warayutt ^{[2
]}

Walalite, Tanapipat ^{[2
]}

Agatsuma, Takeshi ^{[4
]}

Tawong, Wittaya ^{[5
]}

Sithithaworn, Paiboon ^{[6
]}

Andrews, Ross H. ^{[6
,7
]}

Petney, Trevor N. ^{[8
]}

机构：

[1] Thammasat Univ, Fac Med, Dept Preclin Sci, Div Cell Biol, Rangsit Campus, Pathum Thani 12120, Thailand

[2] Mahasarakham Univ, Walai Rukhavej Bot Res Inst, Biodivers & Conservat Res Unit, Maha Sarakham 44150, Thailand

[3] Kasetsart Univ, Fac Forestry, Dept Forest Biol, Bangkok 10900, Thailand

[4] Kochi Univ, Kochi Med Sch, Dept Environm Med, Nankoku, Kochi 7838505, Japan

[5] Naresuan Univ, Fac Agr Nat Resources & Environm, Dept Agr Sci, Phitsanulok 65000, Thailand

[6] Khon Kaen Univ, Fac Med, Cholangiocarcinoma Res Inst, Dept Parasitol, Khon Kaen 40002, Thailand

[7] Imperial Coll London, Fac Med, St Marys Campus,South Wharf St, London W2 1NY, England

[8] State Museum Nat Hist Karlsruhe, Dept Zool & Paleontol & Evolut, Erbprinzenstr 13, D-76133 Karlsruhe, Germany

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 2020年 / 50卷 / 01期

关键词：

Phylogenetic relationships; Genetic diversity; Population genetics; Cholangiocarcinoma; Opisthorchiasis; POPULATION-GENETICS; CLONORCHIS; INFECTION; THAILAND; ECOLOGY; FISH;

D O I：

10.1016/j.ijpara.2019.10.007

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The freshwater snail Bithynia siamensis goniomphalos sensu lato is widely distributed in the Lower Mekong Basin where it acts as the first intermediate host of the liver fluke Opisthorchis viverrini, a group 1 carcinogen causing cholangiocarcinoma. This study explores the genetic structure and geographical variation of B. s. goniomphalos from eight previously studied catchments and eight new catchments. These catchments belong to five previously studied catchment systems and one new catchment system (Tonlesap) in the Lower Mekong Basin. Two new catchment systems, Prachin Buri and Bang Pakong from eastern and central Thailand, respectively, were also examined. We collected 289 specimens of B. s. goniomphalos from 15 previously studied localities and 18 new localities in Thailand, Lao PDR (People's Democratic Republic), and Cambodia. The mitochondrial cytochrome c oxidase subunit 1 and 16S ribosomal DNA sequences were used to determine genetic variation. Classification of haplotypes specified 100 at the cox) locus and 15 at the rrnL locus. Comparison between 16 catchment populations found significant genetic differences (Phi(ST)) between all populations. The phylogenetic tree and haplotype network analyses classified B. s. goniomphalos into three evolutionary lineages (lineage Lineage I contained B. s. goniomphalos from the Mekong, Chi, Mun, Prachin Buri and Bang Pakong catchments in Thailand, including the Nam Ngum catchment in Lao PDR. Lineage II contained all specimens from the Tonlesap catchment, whereas lineage Ill contained specimens from the Mekong and Sea Bang Heang catchments in Thailand and Lao PDR, respectively. Interestingly, Bithynia siamensis siamensis was placed between lineages I and II of B. s. goniomphalos. This study supports the hypothesis that B. s. goniomphalos is a species complex containing at least three distinct evolutionary lineages in the Lower Mekong Basin, and that comprehensive molecular genetic analyses need to be conducted to further our understanding of the evolutionary and systematic relationships of these Bithynia snail taxa. (C) 2019 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.

引用

页码：55 / 62

页数：8

共 2 条

[1] Genetic structure and evidence for coexistence of three taxa of Bithynia (Gastropoda: Bithyniidae), the intermediate host of Opisthorchis viverrini sensu lato (Digenea: Opisthorchiidae) in Thailand examined by mitochondrial DNA sequences analyses
Bunchom, Naruemon
Tantrawatpan, Chairat
Agatsuma, Takeshi
Suganuma, Narufumi
Pilap, Warayutt
Suksavate, Warong
Sithithaworn, Paiboon
Petney, Trevor N.
Andrews, Ross H.
Saijuntha, Weerachai
ACTA TROPICA, 2021, 221
[2] Current assessment of the systematics and population genetics of Opisthorchis viverrini sensu lato (Trematoda: Opisthorchiidae) and its first intermediate host Bithynia siamensis sensu lato (Gastropoda: Bithyniidae) in Thailand and Southeast Asia
Saijuntha, Weerachai
Andrews, Ross H.
Sithithaworn, Paiboon
Petney, Trevor N.
INFECTION GENETICS AND EVOLUTION, 2022, 97

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