Enhanced Recovery of Antioxidant Compounds from Hazelnut (Corylus avellana L.) Involucre Based on Extraction Optimization: Phytochemical Profile and Biological Activities

被引:50
作者
Rusu, Marius Emil [1 ]
Fizesan, Ionel [2 ]
Pop, Anca [2 ]
Gheldiu, Ana-Maria [3 ]
Mocan, Andrei [3 ]
Crisan, Gianina [3 ]
Vlase, Laurian [1 ]
Loghin, Felicia [2 ]
Popa, Daniela-Saveta [2 ]
Tomuta, Ioan [1 ]
机构
[1] Iuliu Hatieganu Univ Med & Pharm, Fac Pharm, Dept Pharmaceut Technol & Biopharmaceut, 8 Victor Babes, Cluj Napoca 400012, Romania
[2] Iuliu Hatieganu Univ Med & Pharm, Fac Pharm, Dept Toxicol, 8 Victor Babes, Cluj Napoca 400012, Romania
[3] Iuliu Hatieganu Univ Med & Pharm, Fac Pharm, Dept Pharmaceut Bot, 8 Victor Babes, Cluj Napoca 400012, Romania
关键词
hazelnut involucre; antioxidants; polyphenols; phytosterols; biological activity; experimental design; LC-MS; turbo-extraction; Ultra-Turrax; enzymatic inhibition; TURKISH TOMBUL HAZELNUT; LYCIUM-BARBARUM L; CHEMICAL-COMPOSITION; BIOACTIVE COMPOUNDS; NUT CONSUMPTION; HEALTH; IDENTIFICATION; LEAVES; MS; CONSTITUENTS;
D O I
10.3390/antiox8100460
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Tree nut by-products could contain a wide range of phytochemicals, natural antioxidants, which might be used as a natural source for dietary supplements. The aim of the present study was to evaluate the phenolic and sterolic composition, as well as the antioxidant and other biological activities, of hazelnut involucre (HI) extracts. Experimental designs were developed in order to select the optimum extraction conditions (solvent, temperature, time) using turbo-extraction by Ultra-Turrax for obtaining extracts rich in bioactive compounds. Qualitative and quantitative analyses were performed by LC-MS and LC-MS/MS and they revealed important amounts of individual polyphenols and phytosterols, molecules with antioxidant potential. The richest polyphenolic HI extract with the highest antioxidant activity by TEAC assay was further evaluated by other in vitro antioxidant tests (DPPH, FRAP) and enzyme inhibitory assays. Additionally, the cytotoxic and antioxidant effects of this extract on two cancerous cell lines and on normal cells were tested. This is the first study to analyze the composition of both hydrophilic and lipophilic bioactive compounds in HI extracts. Our findings reveal that this plant by-product presents strong biological activities, justifying further research, and it could be considered an inexpensive source of natural antioxidants for food, pharmaceutical, or cosmetic industry.
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页数:27
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