Dendrobium nobile Lindl. polysaccharides improve follicular development in PCOS rats

被引：47

作者：

Zhang, Shun ^{[1
]}

Tu, Haoyan ^{[1
]}

Zhu, Jiamin ^{[3
]}

Liang, Aihong ^{[2
]}

Huo, Peng ^{[4
]}

Shan, Ke ^{[3
]}

He, Junyi ^{[3
]}

Zhao, Meng ^{[3
]}

Chen, Xi ^{[2
]}

Lei, Xiaocan ^{[2
]}

机构：

[1] Guilin Med Univ, Dept Reprod Med Ctr, Affiliated Hosp, Guilin 541001, Peoples R China

[2] Univ South China, Clin Anat & Reprod Med Applicat Inst, Dept Histol & Embryol, Hengyang 421001, Peoples R China

[3] Zunyi Med Univ, Sch Basic Med Sci, Zunyi 563000, Guizhou, Peoples R China

[4] Guilin Med Univ, Sch Publ & Hlth, Guilin 541004, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2020年 / 149卷

关键词：

DNLP; PCOS; Estrous cycle; Proliferation; PCNA; Apoptosis; APOPTOSIS;

D O I：

10.1016/j.ijbiomac.2020.01.196

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polycystic ovary syndrome (PCOS) is the most typical and common metabolic abnormalities in women of reproductive age. This study examined the protective effects of Dendrobium nobile Lindl. polysaccharides (DNLP) on ovarian follicular development in letrozole-induced PCOS rats and explored the underlying molecular mechanisms. The PCOS rats showed the increased body weight, serum testosterone and luteinizing hormone levels and insulin resistance. DNLP treatment reduced the body weight, serum testosterone level and insulin resistance, but failed to affect luteinizing hormone level in the PCOS rats. DNLP treatment recovered disrupted estrous cycle in the PCOS rats. DNLP treatment decreased antral follicles and increased the thickness of the granular cell layer. DNLP treatment increased the PCNA mRNA and protein expression levels in the PCOS ovarian tissues, and inhibited cell apoptosis in the PCOS ovarian tissues via regulating apoptosis-related proteins including Bax, Bcl-2 and caspase-3. In summary, this study demonstrated the protective effects of DNLP on the ovaries in the letrozole-induced PCOS rat model. DNLP exerted its protective effects via improving follicular development and inhibiting apoptosis of ovarian granular cells in PCOS rats. This study will provide experimental basis for the future clinical application of DNLP in the treatment of PCOS. (C) 2020 Published by Elsevier B.V.

引用

页码：826 / 834

页数：9

共 33 条

[1]