Rapid improvement of domestication traits in an orphan crop by genome editing

被引：357

作者：

Lemmon, Zachary H. ^{[1
]}

Reem, Nathan T. ^{[2
]}

Dalrymple, Justin ^{[1
]}

Soyk, Sebastian ^{[1
]}

Swartwood, Kerry E. ^{[2
]}

Rodriguez-Leal, Daniel ^{[1
]}

Van Eck, Joyce ^{[2
,3
]}

Lippman, Zachary B. ^{[1
,4
]}

机构：

[1] Cold Spring Harbor Lab, POB 100, Cold Spring Harbor, NY 11724 USA

[2] Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA

[3] Cornell Univ, Sch Integrat Plant Sci, Plant Breeding & Genet Sect, Ithaca, NY USA

[4] Cold Spring Harbor Lab, Howard Hughes Med Inst, Cold Spring Harbor, NY 11724 USA

来源：

NATURE PLANTS | 2018年 / 4卷 / 10期

基金：

美国国家科学基金会;

关键词：

TOMATO; TRANSFORMATION; FRUIT; GENE;

D O I：

10.1038/s41477-018-0259-x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Genome editing holds great promise for increasing crop productivity, and there is particular interest in advancing breeding in orphan crops, which are often burdened by undesirable characteristics resembling wild relatives. We developed genomic resources and efficient transformation in the orphan Solanaceae crop 'groundcherry' (Physalis pruinosa) and used clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein-9 nuclease (Cas9) (CRISPR-Cas9) to mutate orthologues of tomato domestication and improvement genes that control plant architecture, flower production and fruit size, thereby improving these major productivity traits. Thus, translating knowledge from model crops enables rapid creation of targeted allelic diversity and novel breeding germplasm in distantly related orphan crops.

引用

页码：766 / 770

页数：5

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