CSF microRNA Profiling in Alzheimer's Disease: a Screening and Validation Study

被引：45

作者：

Dangla-Valls, Adria ^{[1
,2
]}

Molinuevo, Jose Luis ^{[1
,2
,3
]}

Altirriba, Jordi ^{[4
]}

Sanchez-Valle, Raquel ^{[1
,2
]}

Alcolea, Daniel ^{[5
,6
]}

Fortea, Juan ^{[5
,6
]}

Rami, Lorena ^{[1
,2
]}

Balasa, Mircea ^{[1
,2
]}

Munoz-Garcia, Cristina ^{[1
,2
]}

Ezquerra, Mario ^{[7
]}

Fernandez-Santiago, Ruben ^{[7
]}

Lleo, Alberto ^{[5
,6
]}

Llado, Albert ^{[1
,2
]}

Antonell, Anna ^{[1
,2
]}

机构：

[1] Hosp Clin Barcelona, Alzheimers Dis & Other Cognit Disorders Unit, Neurol Serv, Villarroel 170, E-08036 Barcelona, Spain

[2] Univ Barcelona, Inst Invest Biomed August Pi & Sunyer IDIBAPS, Villarroel 170, E-08036 Barcelona, Spain

[3] Pasqual Maragall Fdn, Barcelonabeta Brain Res Ctr, Wellington 30, Barcelona 08005, Spain

[4] Univ Geneva, Dept Internal Med Specialties, Lab Metab, Fac Med, Geneva, Switzerland

[5] Univ Autonoma Barcelona, Memory Unit, Dept Neurol, Inst Invest Biomed Sant Pau,Hosp Sant Pau, Barcelona, Spain

[6] Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain

[7] Univ Barcelona, Lab Neurodegenerat Disorders, Neurol Serv, Hosp Clin Barcelona,Inst Invest Biomed August Pi, Barcelona, Spain

来源：

MOLECULAR NEUROBIOLOGY | 2017年 / 54卷 / 09期

关键词：

Alzheimer's disease; microRNAs; Biomarkers; Cerebrospinal fluid; miR-125b; miR-222; CEREBROSPINAL-FLUID; DIFFERENTIAL EXPRESSION; RNAS; IDENTIFICATION; ASSOCIATION; BIOMARKERS; VARIANCE; DEMENTIA; BRAIN;

D O I：

10.1007/s12035-016-0106-x

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

MicroRNAs (miRNAs) are short non-coding RNA molecules that regulate gene expression through post-transcriptional repression of target genes. They have been shown to be implicated in the pathophysiology of Alzheimer's disease (AD) and proposed as disease biomarkers. In the present work, we have studied the expression levels of 754 miRNAs in cerebrospinal fluid (CSF) from AD patients and control subjects. We have explored a first screening cohort (N = 20) and selected 12 miRNAs to be further tested in a second independent validation cohort (N = 69). We have found a significant upregulation of miR-222 and miR-125b in AD CSF. Of these, the association of miR-222 with AD is novel and reported here for the first time whereas upregulation of miR-125b has been previously reported in AD brain. Yet we do not find association with other miRNAs which were previously linked to AD. Our results shed light on potential underlying pathophysiological processes of AD and also point out the need for consensus procedures in CSF miRNA detection and data analysis.

引用

页码：6647 / 6654

页数：8

共 47 条

[1]

Alexandrov Peter N, 2012, Int J Biochem Mol Biol, V3, P365

[2] Normalization of real-time quantitative reverse transcription-PCR data: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets [J].