Cloning of the unculturable parasite Pasteuria ramosa and its Daphnia host reveals extreme genotype-genotype interactions

被引:103
作者
Luijckx, Pepijn [1 ]
Ben-Ami, Frida [2 ]
Mouton, Laurence [1 ,3 ,4 ]
Du Pasquier, Louis [1 ]
Ebert, Dieter [1 ]
机构
[1] Univ Basel, Inst Zool, CH-4051 Basel, Switzerland
[2] Tel Aviv Univ, George S Wise Fac Life Sci, Dept Zool, IL-69978 Tel Aviv, Israel
[3] Univ Lyon, F-69000 Lyon, France
[4] Univ Lyon 1, CNRS, Lab Biometrie & Biol Evolut, UMR 5558, F-69622 Villeurbanne, France
关键词
Coevolution; Daphnia magna; host; parasite; Pasteuria ramosa; specificity; GENE-FOR-GENE; EVOLUTIONARY IMPLICATIONS; PATHOGEN SPECIFICITY; FITNESS CONSEQUENCES; MULTIPLE INFECTIONS; VIRULENCE; COEVOLUTION; IMMUNITY; ECOLOGY; MODELS;
D O I
10.1111/j.1461-0248.2010.01561.x
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
P>The degree of specificity in host-parasite interactions has important implications for ecology and evolution. Unfortunately, specificity can be difficult to determine when parasites cannot be cultured. In such cases, studies often use isolates of unknown genetic composition, which may lead to an underestimation of specificity. We obtained the first clones of the unculturable bacterium Pasteuria ramosa, a parasite of Daphnia magna. Clonal genotypes of the parasite exhibited much more specific interactions with host genotypes than previous studies using isolates. Clones of P. ramosa infected fewer D. magna genotypes than isolates and host clones were either fully susceptible or fully resistant to the parasite. Our finding enhances our understanding of the evolution of virulence and coevolutionary dynamics in this system. We recommend caution when using P. ramosa isolates as the presence of multiple genotypes may influence the outcome and interpretation of some experiments.
引用
收藏
页码:125 / 131
页数:7
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