In vivo study of the effects of exogenous hydrogen sulfide on lung mitochondria in acute lung injury in rats

被引:31
作者
Du, Quansheng [1 ,2 ]
Wang, Chao [3 ]
Zhang, Nan [4 ]
Li, Guofeng [5 ]
Zhang, Meng [6 ]
Li, Liping [5 ]
Zhang, Qingzeng [5 ]
Zhang, Jianxin [1 ]
机构
[1] Hebei Med Univ, Dept Pharmacol, Shijiazhuang 050017, Peoples R China
[2] Hebei Gen Hosp, Intens Care Unit, Shijiazhuang 050051, Peoples R China
[3] Hebei Gen Hosp, Clin Res Ctr, Shijiazhuang 050051, Peoples R China
[4] Childrens Hosp Hebei Prov, Dept Pharmacol, Shijiazhuang 050031, Peoples R China
[5] Hebei Ctr Dis Control & Prevent, Dept Pharmacol, Shijiazhuang 050021, Peoples R China
[6] Hebei Med Univ, Hosp 3, Dept chest Surg, Shijiazhuang 050051, Peoples R China
关键词
Acute lung injury; Mitochondria; Lipopolysaccharide; Exogenous hydrogen sulfide; Mitochondrial lipid peroxidation; RESPIRATORY-DISTRESS-SYNDROME; ACUTE-PANCREATITIS; ANIMAL-MODEL; SEPSIS; DYSFUNCTION; APOPTOSIS; INFLAMMATION; SURVIVAL;
D O I
10.1186/1471-2253-14-117
中图分类号
R614 [麻醉学];
学科分类号
100217 ;
摘要
Background: Acute lung injury (ALI) is a serious disease with high incidence in ICU, and impaired mitochondria function plays a significant role in ALI. In this study, we examined the possible roles of exogenous hydrogen sulfide (H2S) in lung mitochondria regulation in ALI rats. Methods: The rat ALI model was induced by an intra-tongue vein Lipopolysaccharide (LPS) injection. We used sodium hydrosulphide (NaHS) as the H2S donor. We randomly divided 40 Sprague-Dawley rats into five groups: control, LPS injury, LPS + low-dose NaHS (0.78 mg.kg(-1)), LPS + middle-dose NaHS (1.56 mg.kg(-1)), and LPS + high-dose NaHS (3.12 mg.kg(-1)). Rats were killed 3 h after NaHS administration. We calculated a semi-quantitative histological index of lung injury assessments and measured the lung wet-to-dry weight ratio. We further analyzed serum for interleukin-1 beta levels using enzyme-linked immunosorbent assays. We observed lung mitochondria ultrastructures with an electron microscope. We examined oxidative stress markers in lung mitochondria and the mitochondrial swelling and activity. We analyzed lung mitochondria and cytosol Cyt-c protein expression using Western blotting. Results: Compared to the control group, the quantitative assessment score index, wet-to-dry weight ratios, and interleukin-1 beta content in the LPS injury group were significantly increased and the mitochondrial ultrastructure damaged. Furthermore, mitochondrial activity, adenosine triphosphatease, superoxide dismutase, glutathione peroxidase, and mitochondrial Cyt-c protein expression were significantly decreased, and malondialdehyde content, mitochondrial swelling, and cytosol Cyt-c protein expression were significantly increased in the LPS injury group compared to the control group. These effects were lessened by NaHS. Conclusion: Exogenous H2S provided a protective effect against ALI by decreasing the mitochondrial lipid peroxidation level and protecting the cell structure in the LPS-induced rat models. Its regulatory effect on lung mitochondria is positively correlated with the dosage.
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页数:12
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