Increased thioredoxin-1 inhibits SSAT expression in MCF-7 human breast cancer cells

被引：21

作者：

Husbeck, B ^{[1
]}

Stringer, DE ^{[1
]}

Gerner, EW ^{[1
]}

Powis, G ^{[1
]}

机构：

[1] Univ Arizona, Arizona Canc Ctr, Tucson, AZ 85724 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 306卷 / 02期

关键词：

thioredoxin-1; spermidine/spermine N-1-acetyltransferase; breast cancer; polyamines; putrescine; Nrf-2/PMF-1;

D O I：

10.1016/S0006-291X(03)00993-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Spermidine/spermine N-1-acetyltransferase (SSAT) regulates polyamine catabolism. Thioredoxin-1 (Trx-1) is a redox protein that is overexpressed in human cancer leading to increased cell proliferation, decreased apoptosis, and decreased patient survival. We report that SSAT mRNA expression is decreased in Trx-1 transfected MCF-7 human breast cancer cells. There is also a decrease in SSAT enzyme activity and lower putrescine levels but no change in spermine or spermidine levels. The expression of SSAT is regulated by the NF-E2-related factor 2 (Nrf-2) and polyamine modulated factor-1 (PMF-1) transcription factor complex. Trx-1 transfected MCF-7 cells showed decreased Nrf-2/PMF-1 DNA binding without a change in Nrf-2 or PMF-1 protein expression. The results suggest that Trx-1 may play a role in the redox regulation of SSAT expression and polyamine homeostasis that could contribute to the biological effects of Trx-1. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：469 / 475

页数：7

共 52 条

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