Reversal of drug resistance in breast carcinoma cells by anti-mdr1 ribozyme regulated by the tumor-specific MUG-1 promoter

被引：26
|
作者：
Gao, Peng [1 ]
Zhou, Geng-Yin
Guo, Ling-Ling
Zhang, Qing-hui
Zhen, Jun-Hui
Fang, Ai-Ju
Lin, Xiao-Yan
机构：
[1] Shandong Univ, Sch Med, Dept Pathol, Jinan 250012, Peoples R China
[2] Soochow Univ, Sch Med, Dept Pathol, Suzhu 515041, Peoples R China
[3] Shandong Provincial Hosp, Dept Pathol, Jinan 250021, Peoples R China
来源：
CANCER LETTERS | 2007年 / 256卷 / 01期
基金：
中国国家自然科学基金;
关键词：
multidrug resistance; reversal; ribozyme; specific expression;
D O I：
10.1016/j.canlet.2007.06.005
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Multidrug resistance (MDR) is a serious obstacle for cancer chemotherapy. The aim of this study was to reverse MDR of breast carcinoma cells specifically by degrading mdr1 mRNA with anti-mdr1 ribozyme. Our strategy was to limit the expression of ribozyme to only breast-derived cells, but not other type of cells. The results showed the recombinant ribozyme pEGFP-RZmuc was expressed in two kinds of breast carcinoma cells, but not in non-breast-derived cancer cells. Evaluation of chemosensitivity showed that a 15-fold reduction in drug resistance for Adriamycin and a 32-fold reduction in drug resistance for Vinblastine were observed in the transfected cells. Our results demonstrate the efficacy and selectivity of pEGFP-RZmuc to reverse MDR in drug resistant breast carcinoma cells in vitro, (C) 2007 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：81 / 89
页数：9
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