The influence of environmental PO2 on hemoglobin oxygen saturation in developing zebrafish Danio rerio

被引:31
作者
Grillitsch, S
Medgyesy, N
Schwerte, T
Pelster, B [1 ]
机构
[1] Univ Innsbruck, Inst Zool & Limnol, A-6020 Innsbruck, Austria
[2] Ctr Mol Biosci, Innsbruck, Austria
关键词
ontogeny; oxygen exchange; circulatory system; heart; zebrafish; Danio rerio;
D O I
10.1242/jeb.01410
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Several studies suggest that during early larval development of lower vertebrates convective blood flow is not essential to supply oxygen to the tissues, but information about the oxygenation status of larvae during the time of cutaneous respiration is still missing. If convective oxygen transport contributes to the oxygen supply to tissues, venous blood in the central circulatory system should be partly deoxygenated, and hyperoxia should increase the oxygen saturation of the hemoglobin. To analyze the changes in hemoglobin oxygen saturation induced by hyperoxic incubation, zebrafish larvae were incubated in a tiny chamber between polytetrafluoroethylene membranes (Teflon), so that the oxygen supply could be rapidly modified. Hemoglobin oxygen saturation was measured in vivo by combining video imaging techniques with a spectrophotometrical analysis of hemoglobin light absorption at specific wavelengths for maximal absorption of oxygenated and deoxygenated blood (413 nm and 431 nm, respectively) under normoxic conditions and after a 10 min period of hyperoxia (P-O2=100 kPa), assuming that at a P-O2 of 100 kPa the hemoglobin is fully saturated. The results demonstrated that red blood cell oxygenation of zebrafish larvae at 4 days post fertilization (d.p.f.), 5 d.p.f. and 12 d.p.f. could be increased by hyperoxia. The data suggest that at the time of yolk sac degradation (i.e. 4 d.p.f. and 5 d.p.f.), when the total surface area of the animal is reduced, bulk diffusion of oxygen may not be sufficient to prevent a partial deoxygenation of the hemoglobin. The decrease in hemoglobin oxygenation observed at 12 d.p.f. confirms earlier studies indicating that at 12-14 d.p.f., convective oxygen transport becomes necessary to ensure oxygen supply to the growing tissues.
引用
收藏
页码:309 / 316
页数:8
相关论文
共 26 条
[1]  
Bagatto B, 2001, J EXP BIOL, V204, P4335
[2]   O2 consumption and heart rate in developing zebrafish (Danio rerio):: influence of temperature and ambient O2 [J].
Barrionuevo, WR ;
Burggren, WW .
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 1999, 276 (02) :R505-R513
[3]   Method for treating and processing whole chick embryos for autoradiography, immunocytochemistry and other techniques [J].
Battiato, NL ;
Paglini, MG ;
Salvarezza, SB ;
Rovasio, RA .
BIOTECHNIC & HISTOCHEMISTRY, 1996, 71 (06) :286-288
[4]  
Chen Jau-Nian, 1997, P7
[5]  
Dalla Via J., 1983, POLAROGRAPHIC OXYGEN, P202
[6]  
GNAIGER E, 1983, POLAROGRAPHIC OXYGEN, P134
[7]   Influence of hypoxia and of hypoxemia on the development of cardiac activity in zebrafish larvae [J].
Jacob, E ;
Drexel, M ;
Schwerte, T ;
Pelster, B .
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 2002, 283 (04) :R911-R917
[8]   ULTRA-STRUCTURE OF EPIDERMIS OF LARVAE OF HERRING (CLUPEA HARENGUS) IN RELATION TO REARING SALINITY [J].
JONES, MP ;
HOLLIDAY, FG ;
DUNN, AEG .
JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM, 1966, 46 (02) :235-&
[9]   STAGES OF EMBRYONIC-DEVELOPMENT OF THE ZEBRAFISH [J].
KIMMEL, CB ;
BALLARD, WW ;
KIMMEL, SR ;
ULLMANN, B ;
SCHILLING, TF .
DEVELOPMENTAL DYNAMICS, 1995, 203 (03) :253-310
[10]   EFFICIENCY AND RATE OF YOLK UTILIZATION BY DEVELOPING EMBRYOS AND LARVAE OF THE PACIFIC SARDINE SARDINOPS-CAERULEA (GIRARD) [J].
LASKER, R .
JOURNAL OF THE FISHERIES RESEARCH BOARD OF CANADA, 1962, 19 (05) :867-875