Combination of multiphoton and reflective confocal imaging of cornea

被引：22
作者：
Chen, Wei-Liang [1 ]
Sun, Yen [1 ]
Lo, Wen [1 ]
Tan, Hsin-Yuan [2 ]
Dong, Chen-Yuan [1 ]
机构：
[1] Natl Taiwan Univ, Dept Phys, Taipei 106, Taiwan
[2] Chang Gung Mem Hosp, Dept Ophthalmol, Linkou 333, Taiwan
来源：
MICROSCOPY RESEARCH AND TECHNIQUE | 2008年 / 71卷 / 02期
关键词：
multiharmonic generation; multiphoton processes; confocal microscopy; three-dimensional microscopy; ophthalmology;
D O I：
10.1002/jemt.20528
中图分类号：
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号：
100101 ;
摘要：
We combine reflective confocal microscopy with multiphoton microscopy to form a minimally invasive technique to observe the cornea. The two imaging modalities allow detection of complementary information from the cornea. The autofluorescence signal shows the cytoplasm of epithelial cells, and the second harmonic generation signal is used to detect collagen, found mostly in the stroma of the cornea. The reflective confocal imaging allows detection of epithelial cells and keratocytes in the stroma. The system is first tested on bovine cornea. Assessment of the result on the bovine eye will be used to evaluate the potential of the system as a technique for in vivo clinical application.
引用
收藏
页码：83 / 85
页数：3
相关论文
共 16 条
[1]   Confocal microscopy of the cornea [J].
Böhnke, M ;
Masters, BR .
PROGRESS IN RETINAL AND EYE RESEARCH, 1999, 18 (05) :553-628
[2]   Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms [J].
Campagnola, PJ ;
Loew, LM .
NATURE BIOTECHNOLOGY, 2003, 21 (11) :1356-1360
[3]   Intravital imaging of cell movement in tumours [J].
Condeelis, J ;
Segall, JE .
NATURE REVIEWS CANCER, 2003, 3 (12) :921-930
[4]   2-PHOTON LASER SCANNING FLUORESCENCE MICROSCOPY [J].
DENK, W ;
STRICKLER, JH ;
WEBB, WW .
SCIENCE, 1990, 248 (4951) :73-76
[5]   In vivo multiphoton microscopy of deep brain tissue [J].
Levene, MJ ;
Dombeck, DA ;
Kasischke, KA ;
Molloy, RP ;
Webb, WW .
JOURNAL OF NEUROPHYSIOLOGY, 2004, 91 (04) :1908-1912
[6]   Three-dimensional confocal microscopy of the living human eye [J].
Masters, BR ;
Böhnke, M .
ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 2002, 4 :69-91
[7]   Three-dimensional confocal microscopy of the human cornea in vivo [J].
Masters, BR ;
Böhnke, M .
OPHTHALMIC RESEARCH, 2001, 33 (03) :125-135
[8]   3-DIMENSIONALLY RESOLVED NAD(P)H CELLULAR METABOLIC REDOX IMAGING OF THE IN-SITU CORNEA WITH 2-PHOTON EXCITATION LASER-SCANNING MICROSCOPY [J].
PISTON, DW ;
MASTERS, BR ;
WEBB, WW .
JOURNAL OF MICROSCOPY, 1995, 178 :20-27
[9]   Two-photon excitation fluorescence microscopy [J].
So, PTC ;
Dong, CY ;
Masters, BR ;
Berland, KM .
ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 2000, 2 :399-429
[10]   Multiphoton fluorescence and second harmonic generation imaging of the structural alterations in keratoconus ex vivo [J].
Tan, Hsin-Yuan ;
Sun, Yen ;
Lo, Wen ;
Lin, Sung-Jan ;
Hsiao, Ching-Hsi ;
Chen, Yeong-Fong ;
Huang, Samuel Chao-Ming ;
Lin, Wei-Chou ;
Jee, Shiou-Hwa ;
Yu, Hsin-Su ;
Dong, Chen-Yuan .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2006, 47 (12) :5251-5259
12