Evaluation of incubation time for dermatophytes cultures

被引：14

作者：

Rezusta, Antonio ^{[1
]}

de la Fuente, Sonia ^{[2
]}

Gilaberte, Yolanda ^{[3
]}

Vidal-Garcia, Matxalen ^{[4
]}

Alcala, Leticia ^{[4
]}

Lopez-Calleja, Ana ^{[4
]}

Angeles Ruiz, Maria ^{[4
]}

Jose Revillo, Maria ^{[4
]}

机构：

[1] Univ Zaragoza, Dept Microbiol, Hosp Miguel Servet, Zaragoza, Spain

[2] Hosp Ernest Lluch, Dept Dermatol, Avda Isabel La Catolica S-N, Calatayud 50009, Zaragoza, Spain

[3] Hosp San Jorge, Dept Dermatol, Huesca, Spain

[4] Hosp Miguel Servet, Dept Microbiolgy, Clin Microbiol Labortory, Zaragoza, Spain

来源：

MYCOSES | 2016年 / 59卷 / 07期

关键词：

dermatophytes; dermatophytosis; dermatophyte carriage; onychomycosis; incubation time; mycoses; DURATION;

D O I：

10.1111/myc.12484

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

In general, it is recommended to incubate dermatophytes cultures for a minimum of 4 weeks. Several aspects of routine fungal cultures should be evaluated in order to implement appropriate and necessary changes. The aim of this study was to determine the optimum incubation time for routine dermatophytes cultures, analysing the time to find first fungal growth by visual observation. We recorded the time when the initial growth was detected for all dermatophyte isolates during a 4-year period. A total of 5459 dermatophyte cultures were submitted to our laboratory. From the total cultures, only 16 (1.42%) isolates were recovered over/after 17 days of incubation and only three dermatophyte species were recovered over 17 days. Fourteen isolates belong to Trichophyton rubrum, one isolate to Trichophyton mentagrophytes complex and one isolate to Epidermophyton floccosum. We concluded that an incubation period of 17 days is enough to establish a microbiological diagnosis of dermatophytosis.

引用

页码：416 / 418

页数：3

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