Optimization of covalent immobilization of Trichoderma reesei cellulase onto modified ReliZyme HA403 and Sepabeads EC-EP supports for cellulose hydrolysis, in buffer and ionic liquids/buffer media

被引：14

作者：

Bilgin, Ramazan ^{[1
]}

Yalcin, M. Serkan ^{[2
]}

Yildirim, Deniz ^{[3
]}

机构：

[1] Cukurova Univ, Fac Sci & Letters, Dept Chem, TR-01330 Adana, Turkey

[2] Mersin Univ, Res & Applicat Ctr, Adv Technol Educ, Mersin, Turkey

[3] Cukurova Univ, Vocat Sch Ceyhan, Adana, Turkey

来源：

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | 2016年 / 44卷 / 05期

关键词：

ionic liquids; ReliZyme HA403; response surface methodology; Sepabeads EC-EP; RESPONSE-SURFACE METHODOLOGY; ASYMMETRIC ACYLATION; STABILITY; LIPASE; STABILIZATION; QUALITY; ACETATE; ENZYME;

D O I：

10.3109/21691401.2015.1024842

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The covalent immobilization of Trichoderma reesei cellulase onto modified ReliZyme HA403 and Sepabeads EC-EP supports were carried out. The optimal immobilization conditions were determined using response surface methodology. The hydrolysis of cellulose using the free and immobilized cellulase preparations in ionic liquids (IL) using cosolvents was investigated. The hydrolytic activities in buffer medium containing 25% (v/v) of 1-butyl-3-methylimidazolium hexafluorophosphate were around 2.6-, 1.6-, and 5.5-fold higher than the activities in buffer medium. The retained initial activities were 32% and 57%, respectively for cellulase preparations immobilized onto Sepabeads EC-EP support and onto modified ReliZyme HA403 support after 5 reuses.

引用

页码：1276 / 1284

页数：9

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