Molecular cartography of the human skin surface in 3D

被引：266

作者：

Bouslimani, Amina ^{[1
,5
]}

Porto, Carla ^{[1
]}

Rath, Christopher M. ^{[1
]}

Wang, Mingxun ^{[7
]}

Guo, Yurong ^{[2
]}

Gonzalez, Antonio ^{[9
,10
]}

Berg-Lyon, Donna ^{[9
,10
]}

Ackermann, Gail ^{[9
,10
]}

Christensen, Gitte Julie Moeller ^{[1
,12
]}

Nakatsuji, Teruaki ^{[3
]}

Zhang, Lingjuan ^{[3
]}

Borkowski, Andrew W. ^{[3
]}

Meehan, Michael J. ^{[1
]}

Dorrestein, Kathleen ^{[1
]}

Gallo, Richard L. ^{[3
]}

Bandeira, Nuno ^{[1
,7
,8
]}

Knight, Rob ^{[2
,7
,9
,10
,11
]}

Alexandrov, Theodore ^{[1
,13
,14
,15
]}

Dorrestein, Pieter C. ^{[1
,4
,6
]}

机构：

[1] Univ Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, La Jolla, CA 92037 USA

[2] Univ Calif San Diego, Sch Med, La Jolla, CA 92037 USA

[3] Univ Calif San Diego, Div Dermatol, La Jolla, CA 92037 USA

[4] Univ Calif San Diego, Dept Biochem, La Jolla, CA 92037 USA

[5] Univ Calif San Diego, Dept Pharmacol, La Jolla, CA 92037 USA

[6] Univ Calif San Diego, Dept Chem, La Jolla, CA 92037 USA

[7] Univ Calif San Diego, Dept Comp Sci & Engn, La Jolla, CA 92093 USA

[8] Univ Calif San Diego, Ctr Computat Mass Spectrometry, La Jolla, CA 92093 USA

[9] Univ Colorado, Dept Chem & Biochem, Boulder, CO 80309 USA

[10] Univ Colorado, BioFrontiers Inst, Boulder, CO 80309 USA

[11] Univ Colorado, Howard Hughes Med Inst, Boulder, CO 80309 USA

[12] Aarhus Univ, Dept Biomed, DK-8000 Aarhus C, Denmark

[13] Univ Bremen, Ctr Ind Math, D-28359 Bremen, Germany

[14] SCiLS GmbH, D-28359 Bremen, Germany

[15] European Mol Biol Lab, D-69117 Heidelberg, Germany

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2015年 / 112卷 / 17期

基金：

美国国家卫生研究院;

关键词：

human skin; 3D mapping; mass spectrometry; 16S rRNA; HUMAN MICROBIOME; NETWORKING; DIVERSITY; IONIZATION; DEFENSINS; DISEASE;

D O I：

10.1073/pnas.1424409112

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The human skin is an organ with a surface area of 1.5-2 m(2) that provides our interface with the environment. The molecular composition of this organ is derived from host cells, microbiota, and external molecules. The chemical makeup of the skin surface is largely undefined. Here we advance the technologies needed to explore the topographical distribution of skin molecules, using 3D mapping of mass spectrometry data and microbial 16S rRNA amplicon sequences. Our 3D maps reveal that the molecular composition of skin has diverse distributions and that the composition is defined not only by skin cells and microbes but also by our daily routines, including the application of hygiene products. The technological development of these maps lays a foundation for studying the spatial relationships of human skin with hygiene, the microbiota, and environment, with potential for developing predictive models of skin phenotypes tailored to individual health.

引用

页码：E2120 / E2129

页数：10

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