TRSP is dispensable for the Plasmodium pre-erythrocytic phase

被引：4

作者：

Costa, David Mendes ^{[1
,2
]}

Sa, Monica ^{[1
,2
]}

Teixeira, Ana Rafaela ^{[1
,2
]}

Loureiro, Ines ^{[1
,2
]}

Thouvenot, Catherine ^{[3
,5
]}

Golba, Sylvain ^{[3
]}

Amino, Rogerio ^{[4
]}

Tavares, Joana ^{[1
,2
]}

机构：

[1] Univ Porto, i3S, P-4200135 Porto, Portugal

[2] Univ Porto, IBMC, P-4200135 Porto, Portugal

[3] Inst Pasteur, Ctr Prod & Infect Anopheles, F-75015 Paris, France

[4] Inst Pasteur, Unit Malaria Infect & Immun, F-75015 Paris, France

[5] Inst Pasteur, Ultrapole, F-75015 Paris, France

来源：

SCIENTIFIC REPORTS | 2018年 / 8卷

关键词：

MALARIA SPOROZOITE; CIRCUMSPOROZOITE PROTEIN; LAYERED FOLD; FALCIPARUM; BINDING; TRAP; MEMBRANE; DOMAIN;

D O I：

10.1038/s41598-018-33398-8

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Plasmodium sporozoites deposited in the skin following a mosquito bite must migrate and invade blood vessels to complete their development in the liver. Once in the bloodstream, sporozoites arrest in the liver sinusoids, but the molecular determinants that mediate this specific homing are not yet genetically defined. Here we investigate the involvement of the thrombospondin-related sporozoite protein (TRSP) in this process using knockout Plasmodium berghei parasites and in vivo bioluminescence imaging in mice. Resorting to a homing assay, trsp knockout sporozoites were found to arrest in the liver similar to control parasites. Moreover, we found no defects in the establishment of infection in mice following inoculation of trsp knockout sporozoites via intravenous and cutaneous injection or mosquito bite. Accordingly, mutant sporozoites were also able to successfully invade hepatocytes in vitro. Altogether, these results suggest TRSP may have a redundant role in the completion of the pre-erythrocytic phase of the malaria parasite. Nonetheless, identifying molecules with paramount roles in this phase could aid in the search for new antigens needed for the design of a protective vaccine against malaria.

引用

页数：9