Noninvasive methodology for the sampling and extraction of DNA from free-ranging Atlantic spotted dolphins (Stenella frontalis)

被引:18
作者
Green, Michelle L. [1 ]
Herzing, Denise L. [1 ]
Baldwin, John D. [1 ]
机构
[1] Florida Atlantic Univ, Dept Biol Sci, Davie, FL 33314 USA
来源
MOLECULAR ECOLOGY NOTES | 2007年 / 7卷 / 06期
关键词
cetaceans; faeces; noninvasive genotyping; Stenella frontalis;
D O I
10.1111/j.1471-8286.2007.01858.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Genetic sampling and molecular investigations are important parts of studying wild populations. However, collecting tissues from free-ranging animals can be difficult or impractical. This study develops a sampling and extraction protocol for template DNA from faecal material collected in a marine environment from small cetaceans. DNA was extracted from faecal material of free-ranging Atlantic spotted dolphins (Stenella frontalis) and subsequently tested for its suitability in molecular investigations by amplifying both mitochondrial and nuclear DNA. The resulting mitochondrial sequences were found to closely match known S. frontalis haplotypes. Three microsatellite loci were amplified and fall within the expected size range for cetaceans. Mother and calf families previously assigned by observation were genetically confirmed using both mitochondrial haplotype and allele sharing between the mother and offspring. The protocol effectively collects and extracts dolphin DNA from faecal samples and enables species identification as well as confirmation of genetic relatedness and should be considered as a noninvasive alternative to current protocols.
引用
收藏
页码:1287 / 1292
页数:6
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