Non-invasive diagnostics of Liberibacter disease on tomatoes using a hand-held Raman spectrometer

被引:46
|
作者
Sanchez, Lee [1 ]
Ermolenkov, Alexei [1 ]
Tang, Xiao-Tian [2 ]
Tamborindeguy, Cecilia [2 ]
Kurouski, Dmitry [1 ,3 ]
机构
[1] Texas A&M Univ, Dept Biochem & Biophys, College Stn, TX 77843 USA
[2] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA
[3] Texas A&M Univ, Inst Quantum Sci & Engn, College Stn, TX 77843 USA
关键词
Raman spectroscopy; Diagnostics; Liberibacter disease; Tomatoes; CAMPESTRIS PV CITRI; SOLANACEARUM; SPECTROSCOPY; IDENTIFICATION; HAPLOTYPE; STRAINS; CANKER; PLANTS; WALLS;
D O I
10.1007/s00425-020-03359-5
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Main conclusion Hand-held Raman spectroscopy can be used for confirmatory, non-invasive and non-destructive detection and identification of two haplotypes of Liberibacter disease on tomatoes. Using this spectroscopic approach, structural changes in carotenoids, xylan, cellulose and pectin that are associ-ated with this bacterial disease can be determined. 'Candidatus Liberibacter solanacearum' (Lso) is a phloem-limited Gram-negative bacterium that infects crops worldwide. In North America, two haplotypes of Lso (LsoA and LsoB) are transmitted by the potato psyllid, Bactericera cockerelli (Sulc), and infect many solanaceous crops such as potato and tomato. Infected plants exhibit chlorosis, severe stunting, leaf cupping, and scorching. Polymerase chain reaction (PCR) and potato tuber frying are commonly used methods for diagnostics of the plant disease caused by Lso. However, they are time-consuming, costly, destructive to the sample, and often not sensitive enough to detect the pathogen in the early infection stage. Raman spectroscopy (RS) is a noninvasive, nondestructive, analytical technique, which probes chemical composition of analyzed samples. In this study, we demonstrate that Lso infection can be diagnosed by non-invasive spectroscopic analysis of tomato leaves three weeks following infection, before the development of aerial symptoms. In combination with chemometric analyses, Raman spectroscopy allows for 80% accurate diagnostics of Liberibacter disease caused by each of the two different haplotypes. This diagnostics approach is portable and sample agnostic, suggesting that it could be utilized for other crops and could be conducted autonomously.
引用
收藏
页数:6
相关论文
共 50 条
  • [41] Explosive vapour/particles detection using SERS substrates and a hand-held Raman detector
    Heleg-Shabtai, Vered
    Zaltsman, Amalia
    Sharon, Mali
    Sharabi, Hagai
    Nir, Ido
    Marder, Dana
    Cohen, Guy
    Ron, Izhar
    Pevzner, Alexander
    RSC ADVANCES, 2021, 11 (42) : 26029 - 26036
  • [42] Chemical Analysis of Iron Meteorites Using a Hand-Held X-Ray Fluorescence Spectrometer
    Gemelli, Maurizio
    D'Orazio, Massimo
    Folco, Luigi
    GEOSTANDARDS AND GEOANALYTICAL RESEARCH, 2015, 39 (01) : 55 - 69
  • [43] Hand-held mass spectrometer for environmentally relevant analytes using a variety of sampling and ionization methods
    Sanders, Nathaniel L.
    Sokol, Ewa
    Perry, Richard H.
    Huang, Guangming
    Noll, Robert J.
    Duncan, Jason S.
    Cooks, R. Graham
    EUROPEAN JOURNAL OF MASS SPECTROMETRY, 2010, 16 (01) : 11 - 20
  • [44] Diagnostics Using Non-Invasive Technologies in Dermatological Oncology
    Soglia, Simone
    Perez-Anker, Javiera
    Lobos Guede, Nelson
    Giavedoni, Priscila
    Puig, Susana
    Malvehy, Josep
    CANCERS, 2022, 14 (23)
  • [45] A Hand-held Non-Robotic Controller of a Manipulator with Cable Guide for Minimally Invasive Surgery
    Kim, Yonghyeok
    Kim, Jeongryul
    Moon, Yonghwan
    Kim, Keri
    2021 18TH INTERNATIONAL CONFERENCE ON UBIQUITOUS ROBOTS (UR), 2021, : 657 - 660
  • [46] Fabrication of Al/Au hybrid SERS substrate using laser engraving for rapid detection of melamine and its analogues by hand-held Raman spectrometer
    Wongwasuratthakul, Puwasit
    Aumpalop, Weerada
    Chakaja, Chaiwat
    Satapornchai, Pemika
    Eiamsamut, Ploypailin
    Somboonsaksri, Pacharamon
    Limwichean, Saksorn
    Botta, Raju
    Nuntawong, Noppadon
    Horprathum, Mati
    Houngkamhang, Nongluck
    MICROCHEMICAL JOURNAL, 2024, 200
  • [47] Rapid Assessment of Quality Parameters in Processing Tomatoes Using Hand-Held and Benchtop Infrared Spectrometers and Multivariate Analysis
    Wilkerson, Elizabeth D.
    Anthon, Gordon E.
    Barrett, Diane M.
    Sayajon, Glynda Fe G.
    Santos, Alejandra M.
    Rodriguez-Saona, Luis E.
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2013, 61 (09) : 2088 - 2095
  • [48] DETECTION OF PLANT-DISEASE GRADIENTS USING A HAND-HELD, MULTISPECTRAL RADIOMETER
    NUTTER, FW
    PHYTOPATHOLOGY, 1987, 77 (04) : 643 - 643
  • [49] CHEMICAL ANALYSIS OF IRON METEORITES USING A HAND-HELD X-RAY FLUORESCENCE SPECTROMETER.
    Gemelli, M.
    D'Orazio, M.
    Folco, L.
    METEORITICS & PLANETARY SCIENCE, 2014, 49 : A132 - A132
  • [50] Non-invasive detection of glucose using Raman spectroscopy
    Kanger, Johannes S.
    de Mul, Frits F.M.
    Otto, C.
    Proceedings of SPIE - The International Society for Optical Engineering, 3570 : 123 - 129