Reliable Enzyme-Linked Immunosorbent Assay for the Determination of Coconut Milk Proteins in Processed Foods

被引：6

作者：

Surojanametakul, Vipa ^{[1
]}

Doi, Hirotoshi ^{[2
]}

Shibata, Haruki ^{[2
]}

Mizumura, Tasuku ^{[2
]}

Takahashi, Toshio ^{[2
]}

Varanyanond, Warunee ^{[1
]}

Wannapinpong, Sirinrat ^{[1
]}

Shoji, Masahiro ^{[2
]}

Ito, Tatsuhiko ^{[2
]}

Tamura, Hirotoshi ^{[3
]}

机构：

[1] Kasetsart Univ, IFRPD, Bangkok 10900, Thailand

[2] Morinaga Inst Biol Sci Inc, Kanazawa Ku, Yokohama, Kanagawa, Japan

[3] Kagawa Univ, Grad Sch Agr Sci, Kagawa, Japan

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2011年 / 59卷 / 06期

关键词：

coconut milk; food allergy; sandwich ELISA; TREE NUT; HAZELNUT PROTEINS; ALLERGY; ANAPHYLAXIS; PREVALENCE; REACTIVITY; PRODUCTS; PEANUT; ELISA;

D O I：

10.1021/jf104067v

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

This study was designed to develop a novel sandwich enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of coconut milk proteins in processed foods. The developed sandwich ELISA was able to detect coconut milk proteins from various coconut milk products and did not show any cross-reactivity with 41 of 42 kinds of popularly used food ingredients, thus reflecting great specificity for coconut milk proteins. In addition, the established ELISA is highly sensitive and allowed the detection of 0.31 mu g/g of coconut milk protein in complex food matrices. This proposed assay could serve as a useful tool for the detection of the presence of hidden coconut milk proteins in processed foods.

引用

页码：2131 / 2136

页数：6

共 26 条

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