Some potentialities and drawbacks of contemporary size-exclusion chromatography

被引：78

作者：

Stulík, K

Pacáková, V

Tichá, M

机构：

[1] Charles Univ, Fac Sci, Dept Analyt Chem, Prague 12840 2, Czech Republic

[2] Charles Univ, Fac Sci, Dept Biochem, Prague 12840 2, Czech Republic

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2003年 / 56卷 / 1-3期

关键词：

chromatography; size exclusion; gel permeation; macromolecules; separation; detection; characterization; PERFORMANCE LIQUID-CHROMATOGRAPHY; LIGHT-SCATTERING DETECTION; MOLECULAR-WEIGHT DETERMINATION; GEL-PERMEATION CHROMATOGRAPHY; TIME-OF-FLIGHT; MASS-SPECTROMETRY; CAPILLARY ELECTROPHORESIS; COAL DERIVATIVES; POLYMERS; MATRIX;

D O I：

10.1016/S0165-022X(03)00053-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The present state of the chromatographic techniques based on differentiation of solutes according to their molecular sizes is briefly surveyed. Attention is centred on high-performance techniques applied to purification and characterization of natural macromolecules, and on discussion of the chromatographic approaches to the determination of the molecular masses and molecular mass distributions of both natural and synthetic polymers. The basic requirements on the selection of the separation system and the experimental conditions are summarized, demonstrated on a few examples and critically evaluated. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：1 / 13

页数：13

共 40 条

[1] ERRORS CAUSED BY FLOWRATE VARIATION IN HIGH-PERFORMANCE SIZE EXCLUSION CHROMATOGRAPHY (GPC) [J].