Liraglutide, a glucagon-like peptide-1 analog, induce autophagy and senescence in HepG2 cells

被引：33

作者：

Krause, Gabriele Catyana ^{[1
]}

Lima, Kelly Goulart ^{[1
]}

Dias, Henrique Bregolin ^{[1
]}

Goncalves da Silva, Elisa Feller ^{[1
]}

Haute, Gabriela Viegas ^{[1
]}

Basso, Bruno Souza ^{[1
]}

Gassen, Rodrigo Benedetti ^{[2
]}

Marczak, Elisa Simon ^{[2
]}

Bach Nunes, Rafaela Sole ^{[1
]}

de Oliveira, Jarbas Rodrigues ^{[1
,2
]}

机构：

[1] Pontificia Univ Catolica Rio Grande do Sul PUCRS, Lab Pesquisa Biofis Celular & Inflamacao, Av Ipiranga 6681,Predio 12,Bloco C, BR-90619900 Porto Alegre, RS, Brazil

[2] Pontificia Univ Catolica Rio Grande do Sul PUCRS, Lab Imunol Clin & Expt, Inst Pesquisas Biomed, Porto Alegre, RS, Brazil

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY | 2017年 / 809卷

关键词：

Liraglutide; HepG2; Hepatocellular carcinoma; Autophagy; Senescence; TGF-beta; 1; PANCREATIC-CANCER CELLS; INHIBITS GROWTH; HEPATOCELLULAR-CARCINOMA; AUGMENTS APOPTOSIS; EXOCRINE PANCREAS; NO EVIDENCE; RECEPTOR; ACTIVATION; MICE; ENDOCRINE;

D O I：

10.1016/j.ejphar.2017.05.015

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

It has been reported that glucagon-like peptide-1 (GLP-1) agents have been associated with both the increased risk of cancer and inhibition of tumor growth and metastases. The aim of this study is to evaluate the effect of liraglutide on hepatocellular carcinoma cells - HepG2. Cytometry was used to evaluate mechanism related to decreased cell proliferation. Nuclear staining and morphometric analysis were also used to verify the process that was taking place after treatment with liraglutide, and in order to better understand the mechanism, TGF-beta 1 was performed. HepG2 cells decreased proliferation after liraglutide treatment without altering oxidative stress levels. Liraglutide was able to induce autophagy and senescence through the increase of TGF-beta 1 which possibly explains the growth decrease. We have demonstrated that liraglutide has an antiproliferative effect in HepG2 cells inducing autophagy and senescence by the increase of TGF-beta 1.

引用

页码：32 / 41

页数：10

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