Morin, a Flavonoid from Moraceae, Induces Apoptosis by Induction of BAD Protein in Human Leukemic Cells

被引：46

作者：

Park, Cheol ^{[1
]}

Lee, Won Sup ^{[2
]}

Go, Se-Il ^{[2
]}

Nagappan, Arulkumar ^{[2
]}

Han, Min Ho ^{[3
]}

Hong, Su Hyun ^{[3
]}

Kim, Gon Sup ^{[4
]}

Kim, Gi Young ^{[5
]}

Kwon, Taeg Kyu ^{[6
]}

Ryu, Chung Ho ^{[7
]}

Shin, Sung Chul ^{[8
]}

Choi, Yung Hyun ^{[3
,9
,10
]}

机构：

[1] Dong Eui Univ, Coll Nat Sci, Dept Mol Biol, Pusan 614714, South Korea

[2] Gyeongsang Natl Univ, Inst Hlth Sci, Sch Med, Dept Internal Med, Jinju 660702, South Korea

[3] Dong Eui Univ, Coll Oriental Med, Dept Biochem, Pusan 614052, South Korea

[4] Gyeongsang Natl Univ, Life Sci Res Inst, Sch Vet Med, Jinju 660701, South Korea

[5] Jeju Natl Univ, Dept Marine Life Sci, Immunobiol Lab, Cheju 690756, South Korea

[6] Keimyung Univ, Sch Med, Dept Immunol, Taegu 704701, South Korea

[7] Gyeongsang Natl Univ, Life Sci Res Inst, Div Appl Life Sci, BK Program 21, Jinju 660701, South Korea

[8] Gyeongsang Natl Univ, Life Sci Res Inst, Dept Chem, Jinju 660701, South Korea

[9] Dong Eui Univ, Antiaging Res Ctr, Pusan 614714, South Korea

[10] Dong Eui Univ, Blue Bio Ind RIC, Pusan 614714, South Korea

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2015年 / 16卷 / 01期

关键词：

morin; apoptosis; BAD; Bcl-xL; leukemia; NF-KAPPA-B; EPITHELIAL-CELLS; CANCER-RISK; PATHWAY; BCL-2; AKT; ACTIVATION; EXPRESSION; PHOSPHORYLATION; SUPPRESSION;

D O I：

10.3390/ijms16010645

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

a Evidence suggests that phytochemicals can safely modulate cancer cell biology and induce apoptosis. Here, we investigated the anti-cancer activity of morin, a flavone originally isolated from members of the Moraceae family in human leukemic cells, focusing on apoptosis. An anti-cancer effect of morin was screened with several human leukemic cell lines. U937 cells were most sensitive to morin, where it induced caspase-dependent apoptosis in a dose-dependent manner. It also induced loss of MMP (Delta Psi m) along with cytochrome c release, down-regulated Bcl-2 protein, and up-regulated BAX proteins. The apoptotic activity of morin was significantly attenuated by Bcl-2 augmentation. In conclusion, morin induced caspase-dependent apoptosis through an intrinsic pathway by upregulating BAD proteins. In addition, Bcl-2 protein expression is also important in morin-induced apoptosis of U937 cells. This study provides evidence that morin might have anticancer properties in human leukemic cells.

引用

页码：645 / 659

页数：15

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