Nobiletin, a citrus flavonoid, down-regulates matrix metalloproteinase-7 (matrilysin) expression in HT-29 human colorectal cancer cells

被引：81

作者：

Kawabata, K ^{[1
]}

Murakami, A ^{[1
]}

Ohigashi, H ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Agr, Div Food Sci & Biotechnol, Kyoto 6068502, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2005年 / 69卷 / 02期

关键词：

matrix metalloproteinase-7; nobiletin; activator protein-1; HT-29;

D O I：

10.1271/bbb.69.307

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Overexpression of matrix metalloproteinases (MMPs) is associated with cancer metastasis. We assessed mRNA expression of MMPs in six human colorectal cancer cell lines and found a considerable level of MMP-7 expression in HT-29 cells. Next, we searched for natural and synthetic compounds that cause a reduction in the production of proMMP-7 protein, and found that nobiletin (NOB), quercetin, valeryl salicylate, and sulindac sulfone demonstrated marked inhibition. Importantly, NOB attenuated proMMP-7 protein and its mRNA expression both concentration- and time-dependently via a reduction of activator protein-1 (AP-1) DNA binding activity, suggesting it as a promising agent for suppression of cancer cell invasion and metastasis through MMP-7 gene repression.

引用

页码：307 / 314

页数：8

共 49 条

[31]

2-E

[32] Up-regulation of TRPM-2, MMP-7 and ID-1 during sex hormone-induced prostate carcinogenesis in the Noble rat [J].

Ouyang, XS ;

Wang, X ;

Lee, DTW ;

Tsao, SW ;

Wong, YC .

CARCINOGENESIS, 2001, 22 (06) :965-973

[33] Expression of gelatinases A and B, stromelysin-3 and matrilysin genes in breast carcinomas: clinico-pathological correlations [J].

Pacheco, MM ;

Mourao, M ;

Mantovani, EB ;

Nishimoto, IN ;

Brentani, MM .

CLINICAL & EXPERIMENTAL METASTASIS, 1998, 16 (07) :577-585

[34] JunB suppresses cell proliferation by transcriptional activation of p16INK4a expression [J].

Passegué, E ;

Wagner, EF .

EMBO JOURNAL, 2000, 19 (12) :2969-2979

[35]

Piazza GA, 1997, CANCER RES, V57, P2909

[36] Evaluation of the effects of swainsonine, captopril, tangeretin and nobiletin on the biological behaviour of brain tumour cells in vitro [J].

Rooprai, HK ;

Kandanearatchi, A ;

Maidment, SL ;

Christidou, M ;

Trillo-Pazos, G ;

Dexter, DT ;

Rucklidge, GJ ;

Widmer, W ;

Pilkington, GJ .

NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY, 2001, 27 (01) :29-39

[37]

Rudolph-Owen LA, 1998, CANCER RES, V58, P5500

[38]

Sato T, 2002, CANCER RES, V62, P1025

[39]

Senota A, 1998, CLIN EXP METASTAS, V16, P313

[40] Activation of PPARα or γ reduces secretion of matrix metalloproteinase 9 but not interleukin 8 from human monocytic THP-1 cells [J].

Shu, H ;

Wong, BM ;

Zhou, GC ;

Li, Y ;

Berger, J ;

Woods, JW ;

Wright, SD ;

Cai, TQ .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 267 (01) :345-349

← 1 2 3 4 5 →