Heterologous vaccination regimens with self-amplifying RNA and adenoviral COVID vaccines induce robust immune responses in mice

被引：95

作者：

Spencer, Alexandra J. ^{[1
]}

McKay, Paul F. ^{[2
]}

Belij-Rammerstorfer, Sandra ^{[1
]}

Ulaszewska, Marta ^{[1
]}

Bissett, Cameron D. ^{[1
]}

Hu, Kai ^{[2
]}

Samnuan, Karnyart ^{[2
]}

Blakney, Anna K. ^{[2
]}

Wright, Daniel ^{[1
]}

Sharpe, Hannah R. ^{[1
]}

Gilbride, Ciaran ^{[1
]}

Truby, Adam ^{[1
]}

Allen, Elizabeth R. ^{[1
]}

Gilbert, Sarah C. ^{[1
]}

Shattock, Robin J. ^{[2
]}

Lambe, Teresa ^{[1
]}

机构：

[1] Univ Oxford, Jenner Inst, Nuffield Dept Med, Oxford, England

[2] Imperial Coll London, Dept Infect Dis, London, England

来源：

NATURE COMMUNICATIONS | 2021年 / 12卷 / 01期

关键词：

CHADOX1; NCOV-19; IMMUNOGENICITY; SAFETY; ADULTS;

D O I：

10.1038/s41467-021-23173-1

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Several vaccines have demonstrated efficacy against SARS-CoV-2 mediated disease, yet there is limited data on the immune response induced by heterologous vaccination regimens using alternate vaccine modalities. Here, we present a detailed description of the immune response, in mice, following vaccination with a self-amplifying RNA (saRNA) vaccine and an adenoviral vectored vaccine (ChAdOx1 nCoV-19/AZD1222) against SARS-CoV-2. We demonstrate that antibody responses are higher in two-dose heterologous vaccination regimens than single-dose regimens. Neutralising titres after heterologous prime-boost were at least comparable or higher than the titres measured after homologous prime boost vaccination with viral vectors. Importantly, the cellular immune response after a heterologous regimen is dominated by cytotoxic T cells and Th1(+) CD4 T cells, which is superior to the response induced in homologous vaccination regimens in mice. These results underpin the need for clinical trials to investigate the immunogenicity of heterologous regimens with alternate vaccine technologies. Heterologous vaccination regimens for COVID-19 could be useful for example if there is a shortage of one vaccine type. Here, Spencer et al. show that heterologous vaccination with a self-amplifying RNA vaccine and an adenoviral vectored vaccine performs at least as well as the homologous vaccinations in mice.

引用

页数：8

共 21 条

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Wang, Sheng-Fan ;

Tseng, Sung-Pin ;

Yen, Chia-Hung ;

Yang, Jyh-Yuan ;

Tsao, Ching-Han ;

Shen, Chun-Wei ;

Chen, Kuan-Hsuan ;

Liu, Fu-Tong ;

Liu, Wu-Tse ;

Chen, Yi-Ming Arthur ;

Huang, Jason C. .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2014, 451 (02) :208-214

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