The zinc finger protein CLAMP promotes long-range chromatin interactions that mediate dosage compensation of the Drosophila male X-chromosome

被引:8
作者
Jordan, William [1 ]
Larschan, Erica [1 ]
机构
[1] Brown Univ, Dept Mol Biol Cellular Biol & Biochem, Providence, RI 02912 USA
关键词
Dosage compensation; Three-dimensional genome organization; Transcription; Chromatin; Drosophila; MSL complex; MSL COMPLEX; HUMAN GENOME; GENES; REVEALS; SEQUENCE; VISUALIZATION; ARCHITECTURE; MOF;
D O I
10.1186/s13072-021-00399-3
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Background Drosophila dosage compensation is an important model system for defining how active chromatin domains are formed. The male-specific lethal dosage compensation complex (MSLc) increases transcript levels of genes along the length of the single male X-chromosome to equalize with that expressed from the two female X-chromosomes. The strongest binding sites for MSLc cluster together in three-dimensional space largely independent of MSLc because clustering occurs in both sexes. CLAMP, a non-sex specific, ubiquitous zinc finger protein, binds synergistically with MSLc to enrich the occupancy of both factors on the male X-chromosome. Results Here, we demonstrate that CLAMP promotes the observed three-dimensional clustering of MSLc binding sites. Moreover, the X-enriched CLAMP protein more strongly promotes longer-range three-dimensional interactions on the X-chromosome than autosomes. Genome-wide, CLAMP promotes three-dimensional interactions between active chromatin regions together with other insulator proteins. Conclusion Overall, we define how long-range interactions which are modulated by a locally enriched ubiquitous transcription factor promote hyper-activation of the X-chromosome to mediate dosage compensation.
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页数:16
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