Chitosan/silk fibroin biomimic scaffolds reinforced by cellulose acetate nanofibers for smooth muscle tissue engineering

被引:32
作者
Zhao, Weiguang [1 ]
Cao, Shiyi [2 ]
Cai, Haoxin [3 ]
Wu, Yang [2 ]
Pan, Qi [1 ]
Lin, Heng [2 ]
Fang, Jun [4 ]
He, Yinyan [5 ,6 ]
Deng, Hongbing [2 ,3 ]
Liu, Zhihong [1 ]
机构
[1] Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Urol, Sch Med, Shanghai 200080, Peoples R China
[2] Wuhan Univ, Sch Resource & Environm Sci, Hubei Key Lab Biomass Resource Chem & Environm Bio, Wuhan 430079, Peoples R China
[3] Donghua Univ, Innovat Ctr Text Sci & Technol, Shanghai 200051, Peoples R China
[4] Shanghai Jiao Tong Univ, Med X Res Inst, Sch Biomed Engn, Shanghai 200240, Peoples R China
[5] Tongji Univ, Shanghai Matern & Infant Hosp 1, Dept Gynecol Oncol, Sch Med, Shanghai 201204, Peoples R China
[6] Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Obstet & Gynecol, Sch Med, Shanghai 200080, Peoples R China
基金
中国国家自然科学基金; 上海市自然科学基金;
关键词
Tissue engineering; Cryogel; Chitosan; Silk fibroin; Nanofiber; Biomimic; DIFFERENTIATION; BIOPOLYMERS; SEPARATION; STIFFNESS; MEMBRANE; MATRIX; CELLS;
D O I
10.1016/j.carbpol.2022.120056
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Reconstruction of multilayered and functional smooth muscle tissues remains a challenge nowadays. Cryogels possess great advantages in three-dimensional tissue regeneration owing to the interconnected macroporous structure, but their applications have been hindered because of limited mechanical properties. Inspired by the natural extracellular matrix, cellulose acetate electrospun nanofibers (NFs) were incorporated to chitosan/silk fibroin (CS/SF) cryogel scaffolds to address this problem in this work. Compared with pure CS/SF scaffolds, CS/ SF/NFs composite scaffolds showed roughened surface and enlarged pore size (216.2 +/- 65.3 mu m vs. 263.1 +/- 75.7 mu m) allowing for cell adhesion and proliferation. Incorporation of nanofibers upgraded the mechanical properties of scaffolds with a six-fold increase in compressive modulus. The proliferation and infiltration of smooth muscle cells (SMCs) were remarkably improved with the existence of nanofibers. Besides, SMCs cultured on CS/SF/NFs scaffolds displayed higher expression of contraction-related genes, verifying their potential for smooth muscle tissue engineering.
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页数:11
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