Food web structure and trophic levels in polyculture rice-crab fields

Stable carbon and nitrogen isotopes were used to investigate nutrient pathways and trophic relationships from the rice-crab system in Panjin, Liaoning Province, China. Values of delta C-13 ranged from -27.38aEuro degrees +/- 0.44aEuro degrees to -18.34aEuro degrees +/- 0.26aEuro degrees and delta N-15 ranged from 1.10aEuro degrees +/- 0.88aEuro degrees to 9.33aEuro degrees +/- 0.57aEuro degrees. Pseudorasbora parva (Stone moroko) had the highest delta C-13 and delta N-15 values. The lowest delta C-13 values were obtained for the macrophytes and the lowest delta N-15 value was found in sediments. Stable carbon and nitrogen isotopes were used to determine the contribution of different food items to the diets of crabs. The delta C-13 results indicated that the Pseudorasbora parva made the greatest contribution to the diet of Eriocheir sinensis (Chinese mitten crab), while the delta N-15 results indicated that most food items contributed more than 10% to the diet of the crab. There were three trophic levels identified in the system (Levels 0-2). The crab Eriocheir sinensis, fish Pseudorasbora parva and Misgurnus anguillicaudatus (Oriental weatherfish), and the oligochaete Limnodrilus hoffmeisteri (Limnodrilus worm), were at the second level, zooplankton were at the first level and suspended particulate matter and macrophytes were at trophic position 0.