Protein Encapsulation: A Nanocarrier Approach to the Fluorescence Imaging of an Enzyme-Based Biomarker

被引：23

作者：

Jia, Zhiyuan ^{[1
,2
]}

Han, Hai-Hao ^{[3
,4
]}

Sedgwick, Adam C. ^{[5
]}

Williams, George T. ^{[6
]}

Gwynne, Lauren ^{[6
]}

Brewster, James T., II ^{[5
]}

Bull, Steven D. ^{[6
]}

Jenkins, A. Toby A. ^{[6
]}

He, Xiao-Peng ^{[3
,4
]}

Schoenherr, Holger ^{[1
,2
]}

Sessler, Jonathan L. ^{[5
]}

James, Tony D. ^{[6
]}

机构：

[1] Univ Siegen, Phys Chem, Dept Chem & Biol, Siegen, Germany

[2] Univ Siegen, Res Ctr Micro & Nanochem & Engn C, Dept Chem & Biol, Siegen, Germany

[3] East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Sch Chem & Mol Engn, Key Lab Adv Mat, Shanghai, Peoples R China

[4] East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Sch Chem & Mol Engn, Joint Int Res Lab Precis Chem & Mol Engn, Shanghai, Peoples R China

[5] Univ Texas Austin, Dept Chem, Austin, TX 78712 USA

[6] Univ Bath, Dept Chem, Bath, Avon, England

来源：

FRONTIERS IN CHEMISTRY | 2020年 / 8卷

基金：

中国国家自然科学基金; 英国工程与自然科学研究理事会; 英国医学研究理事会; 欧洲研究理事会; 美国国家卫生研究院;

关键词：

elastase detection; BSA-based nanocarrier; nanocarrier-based enzyme detection; fluorescence imaging; cell imaging; HUMAN NEUTROPHIL ELASTASE; THERAPEUTIC TARGET; PROBES; SUBSTRATE; DIAGNOSIS; DESIGN;

D O I：

10.3389/fchem.2020.00389

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Here, we report a new pentafluoropropanamido rhodamine fluorescent probe (ACS-HNE) that allows for the selective detection of neutrophil elastase (NE). ACS-HNE displayed high sensitivity, with a low limit of detection (<5.3 nM), and excellent selectivity toward elastase over other relevant biological analytes and enzymes. The comparatively poor solubility and cell permeability of neat ACS-HNE was improved by creating an ACS-HNE-albumin complex; this approach allowed for improvements in thein situvisualization of elastase activity in RAW 264.7 cells relative to ACS-HNE alone. The present study thus serves to demonstrate a simple universal strategy that may be used to overcome cell impermeability and solubility limitations, and to prepare probes suitable for the cellular imaging of enzymatic activityin vitro.

引用

页数：6

共 47 条

[1] A novel method for rapid detection of a Helicobacter pylori infection using a γ-glutamyltranspeptidase-activatable fluorescent probe [J].

Akashi, Taro ;

Isomoto, Hajime ;

Matsushima, Kayoko ;

Kamiya, Mako ;

Kanda, Tsutomu ;

Nakano, Masayuki ;

Onoyama, Takumi ;

Fujii, Masashi ;

Akada, Junko ;

Akazawa, Yuko ;

Ohnita, Ken ;

Takeshima, Fuminao ;

Nakao, Kazuhiko ;

Urano, Yasuteru .

SCIENTIFIC REPORTS, 2019, 9 (1)

[2] Mice lacking neutrophil elastase reveal impaired host defense against gram negative bacterial sepsis [J].

Belaaouaj, A ;

McCarthy, R ;

Baumann, M ;

Gao, ZM ;

Ley, TJ ;

Abraham, SN ;

Shapiro, SD .

NATURE MEDICINE, 1998, 4 (05) :615-618

[3] An Activatable Theranostic for Targeted Cancer Therapy and Imaging** [J].

Bhuniya, Sankarprasad ;

Maiti, Sukhendu ;

Kim, Eun-Joong ;

Lee, Hyunseung ;

Sessler, Jonathan L. ;

Hong, Kwan Soo ;

Kim, Jong Seung .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2014, 53 (17) :4469-4474

[4] SYNTHESIS AND ANALYTICAL USE OF A HIGHLY SENSITIVE AND CONVENIENT SUBSTRATE OF ELASTASE [J].

BIETH, J ;

SPIESS, B ;

WERMUTH, CG .

BIOCHEMICAL MEDICINE, 1974, 11 (04) :350-357

[5] Mammals divert endogenous genotoxic formaldehyde into one-carbon metabolism [J].

Burgos-Barragan, Guillermo ;

Wit, Niek ;

Meiser, Johannes ;

Dingler, Felix A. ;

Pietzke, Matthias ;

Mulderrig, Lee ;

Pontel, Lucas B. ;

Rosado, Ivan V. ;

Brewer, Thomas F. ;

Cordell, Rebecca L. ;

Monks, Paul S. ;

Chang, Christopher J. ;

Vazquez, Alexei ;

Patel, Ketan J. .

NATURE, 2017, 548 (7669) :549-+

[6] RAPID DIAGNOSIS OF MALARIA BY USE OF FLUORESCENT-PROBES [J].

CARAMELLO, P ;

LUCCHINI, A ;

SAVOIA, D ;

GIOANNINI, P .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 1993, 17 (04) :293-297

[7]

Chan J, 2012, NAT CHEM, V4, P973, DOI [10.1038/nchem.1500, 10.1038/NCHEM.1500]

[8] Albumin Carriers for Cancer Theranostics: A Conventional Platform with New Promise [J].

Chen, Qian ;

Liu, Zhuang .

ADVANCED MATERIALS, 2016, 28 (47) :10557-10566

[9] Inclusion complex of fluorescent 4-hydroxycoumarin derivatives with native β-cyclodextrin:: Enhanced stabilization induced by the appended substituent [J].

Dondon, W ;

Fery-Forgues, S .

JOURNAL OF PHYSICAL CHEMISTRY B, 2001, 105 (43) :10715-10722

[10] Autonomously Sensing Hydrogels for the Rapid and Selective Detection of Pathogenic Bacteria [J].

Ebrahimi, Mir-Morteza Sadat ;

Laabei, Maisem ;

Jenkins, A. Tobias A. ;

Schoenherr, Holger .

MACROMOLECULAR RAPID COMMUNICATIONS, 2015, 36 (24) :2123-2128

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