Photosensitized xanthone-based oxidation of guanine and its repair: A laser flash photolysis study

被引：11

作者：

Tang, Ruizhi ^{[1
,2
]}

Zhang, Peng ^{[1
,2
]}

Li, Haixia ^{[1
,2
]}

Liu, Yancheng ^{[1
,2
]}

Wang, Wenfeng ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China

[2] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 2011年 / 105卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Xanthone; Guanine; Ferulic acid; Fast repair; DNA damage; Laser flash photolysis; HYDROXYCINNAMIC ACID-DERIVATIVES; DOUBLE-STRANDED DNA; ELECTRON-TRANSFER; PULSE-RADIOLYSIS; PHENYLPROPANOID GLYCOSIDES; AQUEOUS-SOLUTION; TRIPLET-STATE; OH ADDUCTS; DAMAGE; RADICALS;

D O I：

10.1016/j.jphotobiol.2011.08.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The photosensitized oxidation of guanine (G) by the triplet state of xanthone (XT) and the repair for photo-damaged G(-H)(center dot) by ferulic acid (FCA) were investigated using the laser flash photolysis technique. The rate constants of the reaction of triplet state of XT with G and with FCA were determined as 4.5 x 10(9) and 8.0 x 10(9) L mol(-1)s(-1), respectively. Laser exposure was performed on the N(2)-saturated acetonitrile/water (v/v, 1:1) solution containing G, XT and FCA. The transient absorption spectra indicated that the triplet state of XT first reacted with G predominantly to form the oxidized radical G(-H)(center dot). The radical G(-H)(center dot) was rapidly repaired by FCA, and the rate constant for the repair reaction was determined as 1.1 x 10(9) L mol(-1) s(-1). These results demonstrated that non-enzymatic repair is a feasible method for repairing photosensitized DNA bases oxidation. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：157 / 161

页数：5

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