Expression, purification, crystallization and preliminary X-ray characterization of two crystal forms of stationary-phase survival E protein from Campylobacter jejuni

被引：3
|
作者：
Goncalves, A. M. D. [1 ]
Rego, A. T. [1 ]
Thomaz, M. [1 ,2 ]
Enguita, F. J. [1 ,3 ]
Carrondo, M. A. [1 ]
机构：
[1] Univ Nova Lisboa, Inst Tecnol Quim & Biol, P-2781901 Oeiras, Portugal
[2] Inst Biol Expt & Tecnol, P-2748901 Oeiras, Portugal
[3] Univ Lisbon, Fac Med, Inst Mol Med, P-1649028 Lisbon, Portugal
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2008年 / 64卷
关键词：
D O I：
10.1107/S1744309108003096
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Survival E (SurE) protein from Campylobacter jejuni, a Gram-negative mesophile, has been overexpressed in Escherichia coli as a soluble protein, successfully purified and crystallized in two distinct crystal forms. The first form belongs to space group P2(1)2(1)2(1), with a tetramer in the asymmetric unit and unit-cell parameters a = 80.5, b = 119.0, c = 135.3 angstrom. The second form belongs to space group C2, with unit-cell parameters a = 121.4, b = 47.1, c = 97.8 angstrom, and contains a dimer in the asymmetric unit. Diffraction data have been collected from these crystal forms to 2.5 and 2.95 angstrom resolution, respectively.
引用
收藏
页码：213 / 216
页数：4
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