Synthesis and characterization of β-cyclodextrin/fraxinellone inclusion complex and its influence on interaction with human serum albumin

被引：6
作者：
Yan, Jin [1 ]
Tang, Bin [1 ]
Wu, Di [1 ]
Li, Shanshan [1 ]
Xu, Kailin [1 ]
Ma, Xiaoli [1 ]
Wang, Qing [1 ]
Li, Hui [1 ]
机构：
[1] Sichuan Univ, Coll Chem Engn, Chengdu 610065, Sichuan, Peoples R China
来源：
SPECTROSCOPY LETTERS | 2016年 / 49卷 / 08期
关键词：
beta-cyclodextrin; Fraxinellone; human serum albumin; inclusion complex; spectroscopy; DRUG BINDING-SITES; IMIDAZOLE DERIVATIVES; CYCLODEXTRIN; SPECTROSCOPY; FLUORESCENCE; PHTHALATE; SOLUBILIZATION; FRAXINELLONE; DIFFRACTION; ESTERS;
D O I：
10.1080/00387010.2016.1218898
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
Aqueous solubility is one of the key determinants in developing new chemical entities as drugs. In this study, to improve the solubility of fraxinellone, a novel beta-cyclodextrin/ fraxinellone inclusion complex was prepared and characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, X-ray diffraction, and elemental analysis. Moreover, the influence of inclusion of small molecule by cyclodextrins on its binding with transporter in human body is essential for drug development. For the first time, we report the interaction of fraxinellone and its inclusion complex with human serum albumin. Fluorescence quenching of albumin by fraxinellone and its inclusion complex is a static process through complex formation. The results indicated that beta-cyclodextrin did not affect the binding force and site of fraxinellone to the protein. Furthermore, circular dichroism showed that both fraxinellone and its inclusion complex induced a significant change in the secondary structure of human serum albumin.
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收藏
页码：542 / 550
页数：9
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