Expression of prolyl-hydroxylases PHD-1, 2 and 3 and of the asparagine hydroxylase FIH in non-small cell lung cancer relates to an activated HIF pathway

被引：21

作者：

Giatromanolaki, Alexandra ^{[1
,2
,3
]}

Koukourakis, Michael I. ^{[1
,2
,3
]}

Pezzella, Francesco ^{[4
,5
]}

Turley, Helen ^{[4
,5
]}

Sivridis, Efthimlos ^{[1
,2
,3
]}

Bouros, Demosthenes ^{[1
,2
,3
]}

Bougioukas, George ^{[1
,2
,3
]}

Harris, Adrian L. ^{[4
,5
]}

Gatter, Kevin C. ^{[4
,5
]}

机构：

[1] Democritus Univ Thrace, Dept Pathol, Alexandroupolis 68100, Greece

[2] Democritus Univ Thrace, Dept Radiotherapy Oncol, Alexandroupolis 68100, Greece

[3] Democritus Univ Thrace, Dept Pneumol & Thorac Surg, Alexandroupolis 68100, Greece

[4] John Radcliffe Hosp, Canc Res UK Mol Oncol Lab, Inst Mol Med, Oxford OX3 9DU, England

[5] Univ Oxford, Nuffield Dept, Clin Sci Lab, Oxford OX3 9DU, England

来源：

CANCER LETTERS | 2008年 / 262卷 / 01期

关键词：

lung cancer; hypoxia inducible factors; prolyl-hydroxylases; asparagine hydroxylases; lactate dehydrogenase;

D O I：

10.1016/j.canlet.2007.11.041

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The oxygen sensitive prolyl-hydroxylase domain enzymes (PHDs) and the asparagines hydroxylase (FIH, factor inhibiting HIF) regulate the transcriptional activity of HIFs. We assessed the expression of these enzymes in a series of 73 non-small cell lung carcinomas (NSCLC). A direct association of PHDs with FIH and of the PHDs/FIH with HIFs expression was noted. Thirty three of 73 cases had high HIF/PHD expression, predicting intense HIF activity; 19/73 cases had low H IF and high PHD expression mimicking the normal bronchial pattern-, and 18/73 cases had low HIF/PHD (inactive HIF pathway). High lactate dehydrogenase LDH5 expression was noted in cases with high HIF/PHD phenotype. The value of such a classification in defining different metabolic phenotypes of NSCLC deserves further evaluation. (C) 2007 Elsevier Ireland Ltd. All rights reserved.

引用

页码：87 / 93

页数：7

共 19 条

[11] C2028T polymorphism in exon 12 and dinucleotide repeat polymorphism in intron 13 of the HIF-1α gene define HIF-1α protein expression in non-small cell lung cancer [J].