ABHD12 Knockdown Suppresses Breast Cancer Cell Proliferation, Migration and Invasion

被引：15

作者：

Jun, Semo ^{[1
]}

Kim, Seok Won ^{[2
]}

Lim, Ji-Yeon ^{[1
]}

Park, Seon-Joo ^{[1
]}

机构：

[1] Chosun Univ, Dept Premed Sci, Coll Med, 309 Philmoon Daero, Gwangju 61452, South Korea

[2] Chosun Univ, Dept Neurosurg, Coll Med, Gwangju, South Korea

来源：

ANTICANCER RESEARCH | 2020年 / 40卷 / 05期

基金：

新加坡国家研究基金会;

关键词：

ABHD12; breast cancer; proliferation; migration; invasion; BETA HYDROLASE DOMAIN; ALPHA; 2-ARACHIDONOYLGLYCEROL; METABOLISM; PATHWAYS; ENZYMES; SYSTEM;

D O I：

10.21873/anticanres.14231

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background/Aim: Alpha/beta-hydrolase domain containing 12 (ABHD12) is a serine hydrolase that regulates immunological and neurological mechanisms. This study aimed to elucidate the oncogenic effect of ABHD12 on human breast cancer. Materials and Methods: ABHD12 expression was confirmed in breast cancer tissues and breast cancer cell lines by immunohistochemistry and quantitative RT-PCR. To determine the role of ABHD12, ABHD12 siRNA-suppressed breast cancer cells (MCF7 and MDA-MB-231 cells) were investigated for cell proliferation, migration, and invasion capabilities using MTT assays, EdU assays, colony formation assays, and Boyden chamber assays. Results: Immunohistochemical staining showed a higher ABHD12 expression in breast cancer tissues than in normal tissues. Additionally, ABHD12 knockdown was found to inhibit cell growth, proliferation, migration, and invasion in breast cancer cells. Conclusion: ABHD12 plays a crucial role in cell proliferation, migration, and invasion of breast cancer cells.

引用

页码：2601 / 2611

页数：11

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