Development of new dominant selectable markers for the nonconventional yeasts Ogataea polymorpha and Candida famata

被引：7

作者：

Bratiichuk, Dmytro ^{[1
]}

Kurylenko, Olena ^{[1
]}

Vasylyshyn, Roksolana ^{[1
]}

Zuo, MingXing ^{[1
,2
]}

Kang, Yingqian ^{[2
]}

Dmytruk, Kostyantyn ^{[1
]}

Sibirny, Andriy ^{[1
,3
]}

机构：

[1] Natl Acad Sci Ukraine, Inst Cell Biol, Lvov, Ukraine

[2] Guizhou Med Univ, Educ Dept Guizhou, Key Lab Med Microbiol & Parasitol, Guiyang, Peoples R China

[3] Univ Rzeszow, Dept Microbiol & Mol Genet, Rzeszow, Poland

来源：

YEAST | 2020年 / 37卷 / 9-10期

关键词：

aureobasidin; blasticidin; Candida famata (Candida flareri); mycophenolic acid; Ogataea (Hansenula) polymorpha; selectable markers; AUREOBASIDIN-A; SACCHAROMYCES-CEREVISIAE; TRANSFORMATION SYSTEM; BLASTICIDIN-S; AUR1; GENE; RESISTANCE; CONSTRUCTION; RIBOFLAVIN; ALBICANS; ETHANOL;

D O I：

10.1002/yea.3467

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nonconventional yeast Candida famata and Ogataea polymorpha are interesting organisms for basic and applied studies. O. polymorpha is methylotrophic thermotolerant yeast capable of xylose alcoholic fermentation whereas C. famata is capable of riboflavin overproduction. Still, the new tools for molecular research of these species are needed. The aim of this study was to develop the new dominant selective markers for C. famata and O. polymorpha usable in metabolic engineering experiments. In this work, the BSD gene from Aspergillus terreus coding for blasticidin S deaminase, O. polymorpha AUR1 gene required for sphingolipid synthesis and IMH3 gene, which encodes IMP dehydrogenase, were tested as the new dominant selective marker genes. Our results showed that AUR1 and IMH3 genes could be used as dominant selective markers for O. polymorpha with frequencies of transformation of 40 and 20 transformants per microgram of DNA, respectively. The IMH3 gene was successfully used as the marker for construction of O. polymorpha strains with increased ethanol production from xylose due to overexpression of TAL1, TKL1 and AOX1 genes. The BSD gene from A. terreus, conferring resistance to blasticidin, was found to be efficient for selection of C. famata transformants.

引用

页码：505 / 513

页数：9

共 28 条

[11]

Gonchar MV, 2001, FOOD TECHNOL BIOTECH, V39, P37

[12] Transformation system for prototrophic industrial yeasts using the AUR1 gene as a dominant selection marker [J].

Hashida-Okado, T ;

Ogawa, A ;

Kato, I ;

Takesako, K .

FEBS LETTERS, 1998, 425 (01) :117-122

[13] Isolation and characterization of the aureobasidin A-resistant gene, aur1R, on Schizosaccharomyces pombe:: roles of Aur1p+ in cell morphogenesis [J].

Hashida-Okado, T ;

Yasumoto, R ;

Endo, M ;

Takesako, K ;

Kato, I .

CURRENT GENETICS, 1998, 33 (01) :38-45

[14]

HashidaOkado T, 1996, MOL GEN GENET, V251, P236

[15] THE AUR1 GENE IN SACCHAROMYCES-CEREVISIAE ENCODES DOMINANT RESISTANCE TO THE ANTIFUNGAL AGENT AUREOBASIDIN-A (LY295337) [J].

HEIDLER, SA ;

RADDING, JA .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (12) :2765-2769

[16] In Vitro Evaluation of Novel Compounds against Selected Resistant Pseudomonas aeruginosa Isolates [J].

Housman, Seth T. ;

Sutherland, Christina ;

Nicolau, David P. .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2012, 56 (03) :1646-1649

[17]

Kohler GA, 1997, J BACTERIOL, V179, P2331

[18] Peroxisomes and peroxisomal transketolase and transaldolase enzymes are essential for xylose alcoholic fermentation by the methylotrophic thermotolerant yeast, Ogataea (Hansenula) polymorpha [J].

Kurylenko, Olena O. ;

Ruchala, Justyna ;

Vasylyshyn, Roksolana V. ;

Stasyk, Oleh V. ;

Dmytruk, Olena V. ;

Dmytruk, Kostyantyn V. ;

Sibirny, Andriy A. .

BIOTECHNOLOGY FOR BIOFUELS, 2018, 11

[19] Streptomyces lividans Blasticidin S Deaminase and Its Application in Engineering a Blasticidin S-Producing Strain for Ease of Genetic Manipulation [J].

Li, Li ;

Wu, Jun ;

Deng, Zixin ;

Zabriskie, T. Mark ;

He, Xinyi .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2013, 79 (07) :2349-2357

[20]

Lyzak O.O., 2017, Biotechnology of Yeasts and Filamentous Fungi, P281, DOI [10.1007/978-3-319-58829-2_9, DOI 10.1007/978-3-319-58829-2_9, 10.1007/978-3-319-58829-29, DOI 10.1007/978-3-319-58829-29]

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