Increasing oxytetracycline and enrofloxacin concentrations on the algal growth and sewage purification performance of an algal-bacterial consortia system

Oxytetracycline (OTC) and enrofloxacin (EFX) pollution in surface water are very common. Using the algal bacterial consortia system to remove antibiotics remains to be further studied. In this study, the algal growth and sewage purification performance were studied in an algal-bacterial consortia system with different concentrations of antibiotics. The enzyme activity, malondialdehyde content, chlorophyll-a content, extracellular polysaccharide, and protein content of algae were also tested. It was found that the algal growth was promoted by low-dose antibiotics, 21.83% and 22.11% promotion at 0.1 mg L-1 OTC and EFX, respectively. The nutrients and antibiotics removals of the low-dose groups (OTC <5 mg L-1, EFX <1 mg L-1) were not affected significantly. More than 70% of total organic carbon and total phosphorus, and 97.84-99.76% OTC, 42.68-42.90% EFX were removed in the low-dose groups. However, the algal growth was inhibited, and the nutrients removals performance also declined in the high-concentration groups (10 mg L-1 OTC, 5 mg L-1 EFX). The superoxide dismutase and catalase activity, and malondialdehyde content increased significantly (P < 0.05), indicating the increased activity of reactive oxygen species. In addition, the decreased chlorophyll-a content, thylakoid membrane deformation, starch granules accumulation, and plasmolysis showed that the algal physiological functions were affected. These results showed that the algal-bacterial consortia system was more suitable to treat low-concentration antibiotics and provided basic parameters for the consortia application.