Role of metallothionein isoforms in bone formation processes in rat marrow mesenchymal stem cells in culture

被引：22

作者：

Dohi, Y

Shimaoka, H

Ikeuchi, M

Ohgushi, H

Yonemasu, K

Minami, T ^{[1
]}

机构：

[1] Kinki Univ, Sch Sci & Technol, Dept Life Sci, Higashiosaka, Osaka 5778502, Japan

[2] Natl Inst Adv Ind Sci & Technol, Tissue Engn Res Ctr, Amagasaki, Hyogo 6610974, Japan

[3] Nara Med Univ, Dept Publ Hlth, Kashihara, Nara 6348521, Japan

[4] Nara Med Univ, Dept Oral & Maxillofacial Surg, Kashihara, Nara 6348521, Japan

来源：

BIOLOGICAL TRACE ELEMENT RESEARCH | 2005年 / 104卷 / 01期

关键词：

marrow mesenchymal stem cells; metallothionein isoforms; osteoblast differentiation; dexamethasone; alkaline phosphatase;

D O I：

10.1385/BTER:104:1:057

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Temporal changes in mRNAs for metallothionein (MT) isoforms in subcultures of rat marrow mesenchymal stem cells (MSCs) after treatment with dexamethasone were investigated. Both MT-1. and MT-2 mRNA expression in the cultured MSCs with dexamethasone showed maximum levels at d 1, whereas ALP and osteocalcin mRNAs peaked at d 12. MT-3 mRNA was not detected in the cultured MSCs at any time. The expression level of MT-2 mRNA at d I was 9.4-fold higher than that of MT-1 mRNA. Finally, osteoblast differentiation and mineralization of MSCs at d 14 was inhibited by the addition of a common antisense oligonucleotide for both MT-1 and MT-2 in the culture medium during the first 4 d. The results suggest that the large amounts of MT-2 are produced in the early stage of subculture of MSCs, and this might regulate their differentiation.

引用

页码：57 / 69

页数：13

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