Tyrosol, a phenolic compound, ameliorates hyperglycemia by regulating key enzymes of carbohydrate metabolism in streptozotocin induced diabetic rats

被引:58
作者
Chandramohan, Ramasamy [1 ]
Pari, Leelavinothan [1 ]
Rathinam, Ayyasamy [1 ]
Sheikh, Bashir Ahmad [1 ]
机构
[1] Annamalai Univ, Fac Sci, Dept Biochem & Biotechnol, Annamalainagar 608002, Tamil Nadu, India
关键词
Tyrosol; Wistar rat; Diabetes mellitus; Carbohydrate metabolism; Glucose; OXIDATIVE STRESS; OLIVE OIL; PHOSPHOENOLPYRUVATE CARBOXYKINASE; INSULIN-RESISTANCE; GLYCOGEN-SYNTHASE; P-TYROSOL; ANTIOXIDANT; ACID; MODULATION; ASSAY;
D O I
10.1016/j.cbi.2015.01.026
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The present study was designed to evaluate the effects of tyrosol, a phenolic compound, on the activities of key enzymes of carbohydrate metabolism in the control and streptozotocin-induced diabetic rats. Diabetes mellitus was induced in rats by a single intraperitoneal injection of streptozotocin (40 mg/kg body weight). Experimental rats were administered tyrosol 1 ml intra gastrically at the doses of 5, 10 and 20 mg/kg body weight and glibenclamide 1 ml at a dose of 600 mu g/kg body weight once a day for 45 days. At the end of the experimental period, diabetic control rats exhibited significant (p < 0.05) increase in plasma glucose, glycosylated hemoglobin with significant (p < 0.05) decrease in plasma insulin, total hemoglobin and body weight. The activities of key enzymes of carbohydrate metabolism such as phosphoenolpyruvate carboxykinase, fructose-1,6-bisphosphatase and glucose-6-phosphatase were significantly (p < 0.05) increased and the activities of hexokinase and glucose-6-phosphate dehydrogenase were significantly (p < 0.05) decreased in the liver and kidney of diabetic control rats. Further, antioxidants were lowered in diabetic control rats. A significant (p < 0.05) decline in glycogen level in the liver and muscle and glycogen synthase activity in the liver and a significant (p < 0.05) increase in the activity of liver glycogen phosphorylase were observed in diabetic control rats compared to normal control rats. Oral administration of tyrosol to diabetic rats reversed all the above mentioned biochemical parameters to near normal in a dose dependent manner. Tyrosol at a dose of 20 mg/kg body weight showed the highest significant effect than the other two doses. Immunohistochemical staining of pancreas revealed that tyrosol treated diabetic rats showed increased insulin immunoreactive beta-cells, which confirmed the biochemical findings. The observed results were compared with glibenclamide, a standard oral hypoglycemic drug. The results of the present study suggest that tyrosol decreases hyperglycemia, by its antioxidant effect. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:44 / 54
页数:11
相关论文
共 78 条
[1]   Effects of rosiglitazone and aspirin on experimental model of induced type 2 diabetes in rats: focus on insulin resistance and inflammatory markers [J].
Abdin, Amany A. ;
Baalash, Amal A. ;
Hamooda, Hala E. .
JOURNAL OF DIABETES AND ITS COMPLICATIONS, 2010, 24 (03) :168-178
[2]  
Ahn EY, 2008, ONCOL REP, V19, P527
[3]  
[Anonymous], 2001, Biochemistry.
[4]  
[Anonymous], EXP DIABETES RES
[5]  
[Anonymous], 2013, Diabetes Atlas, V6th edn
[6]  
Aziz A, 2011, EXPERT REV CARDIOVAS, V9, P403, DOI [10.1586/erc.11.20, 10.1586/ERC.11.20]
[7]  
Babu PS, 2007, PHYTOMEDICINE, V14, P15, DOI 10.1016/j.phymed.2006.11.005
[8]  
BAKER H, 1980, NUTR REP INT, V21, P531
[9]   Oxidative stress and inflammatory reaction modulation by white wine [J].
Bertelli, AAE ;
Migliori, M ;
Panichi, V ;
Longoni, B ;
Origlia, N ;
Ferretti, A ;
Cuttano, MG ;
Giovannini, L .
ALCOHOL AND WINE IN HEALTH AND DISEASE, 2002, 957 :295-301
[10]   Effect of Kombucha, a fermented black tea in attenuating oxidative stress mediated tissue damage in alloxan induced diabetic rats [J].
Bhattacharya, Semantee ;
Gachhui, Ratan ;
Sil, Parames C. .
FOOD AND CHEMICAL TOXICOLOGY, 2013, 60 :328-340