Anticancer Activity of Ocimum basilicum and the Effect of Ursolic Acid on the Cytoskeleton of MCF-7 Human Breast Cancer Cells

被引：0

作者：

Qamar, Kehkashan Arshad ^{[2
]}

Dar, Ahsana ^{[1
,2
]}

Siddiqui, Bina S. ^{[1
]}

Kabir, Nurul ^{[2
]}

Aslam, Huma ^{[1
]}

Ahmed, Shakil ^{[3
]}

Erum, Shaista ^{[3
]}

Habib, Shazia ^{[1
]}

Begum, Sabira ^{[1
]}

机构：

[1] Univ Karachi, HEJ Res Inst Chem, Int Ctr Chem & Biol Sci, Karachi 75270, Pakistan

[2] Univ Karachi, Dr Panjwani Ctr Mol Med & Drug Res, Int Ctr Chem & Biol Sci, Karachi 75270, Pakistan

[3] Univ Karachi, Ind Analyt Ctr, Int Ctr Chem & Biol Sci, Karachi 75270, Pakistan

来源：

LETTERS IN DRUG DESIGN & DISCOVERY | 2010年 / 7卷 / 10期

关键词：

Anticancer; Cytoskeleton; MCF-7 cell line; Ocimum basilicum; Ursolic acid; NEURONAL GROWTH CONES; ACTIN-FILAMENTS; LINES; MICROTUBULES; APOPTOSIS; DRUGS; CHEMOTHERAPY; VINBLASTINE; INHIBITION; ACTIVATION;

D O I：

暂无

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The anticancer activity of Ocimum basilicum extract and its fractions was evaluated using human cancer cell lines; active compound(s) residing in it were identified and mechanism of their anti-proliferative action was explored. Methanolic extract was fractionated into petroleum ether soluble (PE-S) and insoluble (PE-I) fractions. These were evaluated on HT-144, MCF-7, NCI-H460 and SF-268 cell lines using Sulforhodamine B assay. Immunofluorescence microscopy was employed to study their effects on the cytoskeleton and nuclei of MCF-7 cells. Fractionation of PE-I (GI(50): 5 mu g/ml; LC50: 71 mu g/ml against MCF-7) led to the isolation of four compounds, mainly ursolic acid (LC50: 18.6 mu g/ml). Ursolic acid (100 mu M) induced a significant decrease in the percentage of cells in anaphase/telophase stages along with F-actin aggregation and mitotic spindle distortion. These results support anti-proliferative activity of O. basilicum extract against MCF-7 cells which may partly be due to effects of ursolic acid on F-actin and microtubules.

引用

页码：726 / 736

页数：11