Cytokinesis is not controlled by calmodulin or myosin light chain kinase in the Caenorhabditis elegans early embryo

被引：14

作者：

Batchelder, Ellen L.

Thomas-Virnig, Christina L.

Hardin, Jeffery D.

White, John G.

机构：

[1] Univ Wisconsin, Mol Biol Lab, Madison, WI 53706 USA

[2] Univ Wisconsin, Dept Anat, Madison, WI 53706 USA

[3] Univ Wisconsin, Dept Pathol & Lab Med, Madison, WI 53706 USA

[4] Univ Wisconsin, Dept Zool, Madison, WI 53706 USA

来源：

FEBS LETTERS | 2007年 / 581卷 / 22期

关键词：

calmodulin; cytokinesis; MLCK; phosphorylation; Caenorhabditis elegans;

D O I：

10.1016/j.febslet.2007.08.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Furrow ingression in animal cell cytokinesis is controlled by phosphorylation of myosin II regulatory light chain (mRLC). In Caenorhabditis elegans embryos, Rho-dependent Kinase (RhoK) is involved in, but not absolutely required for, this phosphorylation. The calmodulin effector myosin light chain kinase (MLCK) can also phosphorylate mRLC and is widely regarded as a candidate for redundant function with RhoK. However, our results show that RNA mediated interference against C. elegans calmodulin and candidate MLCKs had no effect on cytokinesis in wild-type or RhoK mutant embryos, ruling out the calmodulin/MLCK pathway as the missing regulator of cytokinesis in the C elegans early embryo. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V.. All rights reserved.

引用

页码：4337 / 4341

页数：5

共 33 条

[1] The endoplasmic reticulum: a multifunctional signaling organelle [J].