Severity of Idiopathic Scoliosis Is Associated with Differential Methylation: An Epigenome-Wide Association Study of Monozygotic Twins with Idiopathic Scoliosis

被引：10

作者：

Carry, Patrick M. ^{[1
,2
]}

Terhune, Elizabeth A. ^{[2
]}

Trahan, George D. ^{[3
]}

Vanderlinden, Lauren A. ^{[4
]}

Wethey, Cambria, I ^{[2
]}

Ebrahimi, Parvaneh ^{[5
]}

McGuigan, Fiona ^{[5
]}

Akesson, Kristina ^{[5
,6
]}

Hadley-Miller, Nancy ^{[1
,2
]}

机构：

[1] Childrens Hosp Colorado, Musculoskeletal Res Ctr, Aurora, CO 80045 USA

[2] Univ Colorado, Dept Orthoped, Anschutz Med Campus, Aurora, CO 80045 USA

[3] Univ Colorado, Dept Pediat, Anschutz Med Campus, Aurora, CO 80045 USA

[4] Colorado Sch Publ Hlth, Dept Biostat & Informat, Aurora, CO 80045 USA

[5] Lund Univ, Clin & Mol Osteoporosis Res Unit, Clin Sci Malmo, S-20502 Malmo, Sweden

[6] Skane Univ Hosp, Dept Orthoped, S-20502 Malmo, Sweden

来源：

GENES | 2021年 / 12卷 / 08期

关键词：

idiopathic scoliosis; monozygotic twin; epigenome-wide association study; DNA methylation; bone; discordant; curve severity; differentially methylated region; GENETIC EPIDEMIOLOGY; EXTRACELLULAR-MATRIX; CANDIDATE REGIONS; PECTUS EXCAVATUM; DNA METHYLATION; RARE VARIANTS; GPR126; GENE; LBX1; LOCUS; SUSCEPTIBILITY; RS11190870;

D O I：

10.3390/genes12081191

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Epigenetic mechanisms may contribute to idiopathic scoliosis (IS). We identified 8 monozygotic twin pairs with IS, 6 discordant (Cobb angle difference > 10 degrees) and 2 concordant (Cobb angle difference <= 2 degrees). Genome-wide methylation in blood was measured with the Infinium HumanMethylation EPIC Beadchip. We tested for differences in methylation and methylation variability between discordant twins and tested the association between methylation and curve severity in all twins. Differentially methylated region (DMR) analyses identified gene promoter regions. Methylation at cg12959265 (chr. 7 DPY19L1) was less variable in cases (false discovery rate (FDR) = 0.0791). We identified four probes (false discovery rate, FDR < 0.10); cg02477677 (chr. 17, RARA gene), cg12922161 (chr. 2 LOC150622 gene), cg08826461 (chr. 2), and cg16382077 (chr. 7) associated with curve severity. We identified 57 DMRs where hyper- or hypo-methylation was consistent across the region and 28 DMRs with a consistent association with curve severity. Among DMRs, 21 were correlated with bone methylation. Prioritization of regions based on methylation concordance in bone identified promoter regions for WNT10A (WNT signaling), NPY (regulator of bone and energy homeostasis), and others predicted to be relevant for bone formation/remodeling. These regions may aid in understanding the complex interplay between genetics, environment, and IS.

引用

页数：20