SWR1 Chromatin-Remodeling Complex Subunits and H2A.Z Have Non-overlapping Functions in Immunity and Gene Regulation in Arabidopsis

被引:65
作者
Berriri, Souha [1 ]
Gangappa, Sreeramaiah N. [1 ]
Kumar, S. Vinod [1 ]
机构
[1] John Innes Ctr, Cell & Dev Biol Dept, Norwich NR4 7UH, Norfolk, England
基金
英国生物技术与生命科学研究理事会;
关键词
arabidopsis; SWR1; chromatin remodeling; H2A.Z; immunity; gene regulation; WRKY TRANSCRIPTION FACTORS; DISEASE-RESISTANCE; SALICYLIC-ACID; BACTERIAL-RESISTANCE; PLANT-DISEASE; DEFENSE; REPRESSION; EXPRESSION; LOCUS; TEMPERATURE;
D O I
10.1016/j.molp.2016.04.003
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Incorporation of the histone variant H2A.Z into nucleosomes by the SWR1 chromatin remodeling complex is a critical step in eukaryotic gene regulation. In Arabidopsis, SWR1c and H2A.Z have been shown to control gene expression underlying development and environmental responses. Although they have been implicated in defense, the specific roles of the complex subunits and H2A.Z in immunity are not well understood. In this study, we analyzed the roles of the SWR1c subunits, PHOTOPERIOD-INDEPENDENT EARLY FLOWERING1 (PIE1), ACTIN-RELATED PROTEIN6 (ARP6), and SWR1COMPLEX 6 (SWC6), as well as H2A.Z, in defense and gene regulation. We found that SWR1c components play different roles in resistance to different pathogens. Loss of PIE1 and SWC6 function as well as depletion of H2A.Z led to reduced basal resistance, while loss of ARP6 fucntion resulted in enhanced resistance. We found that mutations in PIE1 and SWC6 resulted in impaired effector-triggered immunity. Mutation in SWR1c components and H2A.Z also resulted in compromised jasmonic acid/ethylene-mediated immunity. Genome-wide expression analyses similarly reveal distinct roles for H2A.Z and SWR1c components in gene regulation, and suggest a potential role for PIE1 in the regulation of the cross talk between defense signaling pathways. Our data show that although they are part of the same complex, Arabidopsis SWR1c components could have non-redundant functions in plant immunity and gene regulation.
引用
收藏
页码:1051 / 1065
页数:15
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