The First Identified Nucleocytoplasmic Shuttling Herpesviral Capsid Protein: Herpes Simplex Virus Type 1 VP19C

被引：15
|
作者：
Zhao, Lei [1 ,2 ]
Zheng, Chunfu [1 ]
机构：
[1] Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan, Peoples R China
[2] NW A&F Univ, Div Sci Res, Yangling, Shaanxi, Peoples R China
来源：
PLOS ONE | 2012年 / 7卷 / 08期
基金：
中国国家自然科学基金;
关键词：
NUCLEAR EXPORT SIGNAL; INTRACELLULAR-LOCALIZATION; SEQUENCE; SPECIFICITY; RECEPTOR; IMPORT; CRM1;
D O I：
10.1371/journal.pone.0041825
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
VP19C is a structural protein of herpes simplex virus type 1 viral particle, which is essential for assembly of the capsid. In this study, a nuclear export signal (NES) of VP19C is for the first time identified and mapped to amino acid residues 342 to 351. Furthermore, VP19C is demonstrated to shuttle between the nucleus and the cytoplasm through the NES in a chromosomal region maintenance 1 (CRM1)-dependent manner involving RanGTP hydrolysis. This makes VP19C the first herpesviral capsid protein with nucleocytoplasmic shuttling property and adds it to the list of HSV-1 nucleocytoplasmic shuttling proteins.
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页数：5
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