Early changes in mRNA populations in leaves of ultraviolet-B-treated European beech (Fagus sylvatica L.) seedlings

被引：3
|
作者：
Ernst, D [1 ]
Liegl, I [1 ]
Kiefer, E [1 ]
Seidlitz, HK [1 ]
Heller, W [1 ]
Sandermann, H [1 ]
机构：
[1] GSF, Natl Res Ctr Environm & Hlth, Inst Biochem Plant Pathol, D-85764 Neuherberg, Germany
来源：
ACTA PHYSIOLOGIAE PLANTARUM | 2001年 / 23卷 / 03期
关键词：
European beech; mRNA; ribosomal protein; UV-B;
D O I：
10.1007/s11738-001-0042-9
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Changes in the patterns of gene expression were analyzed in ultraviolet-B (UV-B; 290-320 nm) -treated seedlings of European beech by differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) of mRNA. Utilizing 12 different downstream primers and 27 degenerated 10-mer upstream primers 50 induced and 29 repressed cDNA bands were detected. Nineteen bands were subcloned and sequenced. Two clones revealed significant homologies to known database sequences: putative nascent polypepetide-associated complex alpha and 60S ribosomal protein L37. However, only the latter was confirmed to be UV-B-induced by northern blot analysis.
引用
收藏
页码：343 / 349
页数：7
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