Early changes in mRNA populations in leaves of ultraviolet-B-treated European beech (Fagus sylvatica L.) seedlings

被引：3

作者：

Ernst, D ^{[1
]}

Liegl, I ^{[1
]}

Kiefer, E ^{[1
]}

Seidlitz, HK ^{[1
]}

Heller, W ^{[1
]}

Sandermann, H ^{[1
]}

机构：

[1] GSF, Natl Res Ctr Environm & Hlth, Inst Biochem Plant Pathol, D-85764 Neuherberg, Germany

来源：

ACTA PHYSIOLOGIAE PLANTARUM | 2001年 / 23卷 / 03期

关键词：

European beech; mRNA; ribosomal protein; UV-B;

D O I：

10.1007/s11738-001-0042-9

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Changes in the patterns of gene expression were analyzed in ultraviolet-B (UV-B; 290-320 nm) -treated seedlings of European beech by differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) of mRNA. Utilizing 12 different downstream primers and 27 degenerated 10-mer upstream primers 50 induced and 29 repressed cDNA bands were detected. Nineteen bands were subcloned and sequenced. Two clones revealed significant homologies to known database sequences: putative nascent polypepetide-associated complex alpha and 60S ribosomal protein L37. However, only the latter was confirmed to be UV-B-induced by northern blot analysis.

引用

页码：343 / 349

页数：7

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