Photocrosslinkable diazoresin/pectin films - Synthesis and application as cell culture supports

被引：26
作者：
Plewa, Anna [1 ]
Niemiec, Wiktor [1 ]
Filipowska, Joanna [2 ]
Osyczka, Anna M. [2 ]
Lach, Radoslaw [3 ]
Szczubialka, Krzysztof [1 ]
Nowakowska, Maria [1 ]
机构：
[1] Jagiellonian Univ, Fac Chem, PL-30060 Krakow, Poland
[2] Jagiellonian Univ, Fac Biol & Earth Sci, Dept Cell Biol & Imaging, PL-30060 Krakow, Poland
[3] AGH Univ Sci & Technol, Fac Mat Sci & Ceram, PL-30059 Krakow, Poland
来源：
EUROPEAN POLYMER JOURNAL | 2011年 / 47卷 / 08期
关键词：
Cell culture; Diazoresin; Layer-by-layer method; Pectin; Photocrosslinking; Zeta potential; MESENCHYMAL STEM-CELLS; POLYELECTROLYTE MULTILAYERS; ULTRATHIN FILMS; PECTIN; FABRICATION; CHARGE; DIFFERENTIATION; POLYANILINE; MODULATION; POLYMERS;
D O I：
10.1016/j.eurpolymj.2011.06.002
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
Multilayer polymeric films composed of diphenylamine-4-diazoresin (DR), a synthetic polycation, and pectin (Pec), a polyanionic natural polysaccharide, were obtained using a layer-by-layer method (LbL). The films was photocrosslinked by the irradiation with 350 nm light which resulted in their stabilization against different solvents. The nanostructure of the films was studied using AFM microscopy. Irradiation of the films resulted also in the change of the zeta potential of both DR and Pec outermost layer to more negative values and in the increase of the film hydrophobicity as found from the contact angle measurements. The DR/Pec films have been shown to support cell growth and osteogenic potential of cell culture supports in tissue engineering, as found from the experiments with human bone marrow stromal cells (hMSCs). (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1503 / 1513
页数：11
相关论文
共 60 条
[41]  
Semenov OV, 2009, TISSUE ENG PT A, V15, P2977, DOI [10.1089/ten.tea.2008.0602, 10.1089/ten.TEA.2008.0602]
[42]   Preparation of the layer-by-layer deposited ultrathin film based on the charge-transfer interaction [J].
Shimazaki, Y ;
Mitsuishi, M ;
Ito, S ;
Yamamoto, M .
LANGMUIR, 1997, 13 (06) :1385-1387
[43]   An array of possibilities for pectin [J].
Sorensen, Iben ;
Pedersen, Henriette Lodberg ;
Willats, William G. T. .
CARBOHYDRATE RESEARCH, 2009, 344 (14) :1872-1878
[44]   Stabilization of glucose oxidase in alginate microspheres with photoreactive diazoresin nanofilm coatings [J].
Srivastava, R ;
Brown, JQ ;
Zhu, HG ;
McShane, MJ .
BIOTECHNOLOGY AND BIOENGINEERING, 2005, 91 (01) :124-131
[45]   CONTROLLED GROWTH OF MONODISPERSE SILICA SPHERES IN MICRON SIZE RANGE [J].
STOBER, W ;
FINK, A ;
BOHN, E .
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1968, 26 (01) :62-&
[46]   Molecular-level processing of conjugated polymers .4. Layer-by-layer manipulation of polyaniline via hydrogen-bonding interactions [J].
Stockton, WB ;
Rubner, MF .
MACROMOLECULES, 1997, 30 (09) :2717-2725
[47]   Fabrication of a covalently attached multilayer via photolysis of layer-by-layer self-assembled films containing diazo-resins [J].
Sun, JQ ;
Wu, T ;
Sun, YP ;
Wang, ZQ ;
Zhang, X ;
Shen, JC ;
Cao, WX .
CHEMICAL COMMUNICATIONS, 1998, (17) :1853-1854
[48]   Tuning compliance of nanoscale polyelectrolyte multilayers to modulate cell adhesion [J].
Thompson, MT ;
Berg, MC ;
Tobias, IS ;
Rubner, MF ;
Van Vliet, KJ .
BIOMATERIALS, 2005, 26 (34) :6836-6845
[49]   Antiproliferative and Proapoptotic Actions of Okra Pectin on B16F10 Melanoma Cells [J].
Vayssade, Muriel ;
Sengkhamparn, Nipaporn ;
Verhoef, Rene ;
Delaigue, Claire ;
Goundiam, Oumou ;
Vigneron, Pascale ;
Voragen, Alphons G. J. ;
Schols, Henk A. ;
Nagel, Marie-Danielle .
PHYTOTHERAPY RESEARCH, 2010, 24 (07) :982-989
[50]   If homogalacturonan were a side chain of rhamnogalacturonan I. Implications for cell wall architecture [J].
Vincken, JP ;
Schols, HA ;
Oomen, RJFJ ;
McCann, MC ;
Ulvskov, P ;
Voragen, AGJ ;
Visser, RGF .
PLANT PHYSIOLOGY, 2003, 132 (04) :1781-1789
123456