Photocrosslinkable diazoresin/pectin films - Synthesis and application as cell culture supports

被引：26

作者：

Plewa, Anna ^{[1
]}

Niemiec, Wiktor ^{[1
]}

Filipowska, Joanna ^{[2
]}

Osyczka, Anna M. ^{[2
]}

Lach, Radoslaw ^{[3
]}

Szczubialka, Krzysztof ^{[1
]}

Nowakowska, Maria ^{[1
]}

机构：

[1] Jagiellonian Univ, Fac Chem, PL-30060 Krakow, Poland

[2] Jagiellonian Univ, Fac Biol & Earth Sci, Dept Cell Biol & Imaging, PL-30060 Krakow, Poland

[3] AGH Univ Sci & Technol, Fac Mat Sci & Ceram, PL-30059 Krakow, Poland

来源：

EUROPEAN POLYMER JOURNAL | 2011年 / 47卷 / 08期

关键词：

Cell culture; Diazoresin; Layer-by-layer method; Pectin; Photocrosslinking; Zeta potential; MESENCHYMAL STEM-CELLS; POLYELECTROLYTE MULTILAYERS; ULTRATHIN FILMS; PECTIN; FABRICATION; CHARGE; DIFFERENTIATION; POLYANILINE; MODULATION; POLYMERS;

D O I：

10.1016/j.eurpolymj.2011.06.002

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Multilayer polymeric films composed of diphenylamine-4-diazoresin (DR), a synthetic polycation, and pectin (Pec), a polyanionic natural polysaccharide, were obtained using a layer-by-layer method (LbL). The films was photocrosslinked by the irradiation with 350 nm light which resulted in their stabilization against different solvents. The nanostructure of the films was studied using AFM microscopy. Irradiation of the films resulted also in the change of the zeta potential of both DR and Pec outermost layer to more negative values and in the increase of the film hydrophobicity as found from the contact angle measurements. The DR/Pec films have been shown to support cell growth and osteogenic potential of cell culture supports in tissue engineering, as found from the experiments with human bone marrow stromal cells (hMSCs). (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：1503 / 1513

页数：11

共 60 条

[1] Influence of film architecture on the charge-transfer reactions of metallophthalocyanine layer-by-layer films [J].