Evaluation of Vitek MS for Differentiation of Cryptococcus neoformans and Cryptococcus gattii Genotypes

被引：12

作者：

Machado Siqueira, Lumena P. ^{[1
]}

Favero Gimenes, Viviane M. ^{[1
]}

de Freitas, Roseli Santos ^{[1
]}

Carvalho Melhem, Marcia de Souza ^{[2
]}

Bonfietti, Lucas Xavier ^{[2
]}

da Silva Jr, Afonso Rafael ^{[3
]}

Souza Santos, Leticia B. ^{[1
]}

Motta, Adriana L. ^{[3
]}

Rossi, Flavia ^{[3
]}

Benard, Gil ^{[1
]}

de Almeida Jr, Joao N. ^{[1
,3
]}

机构：

[1] Univ Sao Paulo, Inst Med Trop, Lab Micol Med LIM 53, Sao Paulo, Brazil

[2] Adolfo Lutz Inst, Secao Micol, Div Tecn Biol Med, Sao Paulo, Brazil

[3] Univ Sao Paulo, Fac Med, Hosp Clin, Lab Cent LIM 03, Sao Paulo, Brazil

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2019年 / 57卷 / 01期

关键词：

Cryptococcus gattii; Cryptococcus neoformans; MALDI-TOF; VITEK MS; genotypic identification; DESORPTION IONIZATION-TIME; FLIGHT MASS-SPECTROMETRY; MOLECULAR TYPES; IDENTIFICATION; COMPLEX; IVD;

D O I：

10.1128/JCM.01282-18

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Cryptococcus neoformans and Cryptococcus gattii are the main pathogenic species of invasive cryptococcosis among the Cryptococcus species. Taxonomic studies have shown that these two taxa have different genotypes or molecular types with biological and ecoepidemiological peculiarities. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been proposed as an alternative method for labor-intensive methods for C. neoformans and C. gattii genotype differentiation. However, Vitek MS, one of the commercial MALDI-TOF MS instruments, has not been yet been evaluated for this purpose. Thus, we constructed an in-house database with reference strains belonging to the different C. neoformans (VNI, VNII, VNIII, and VNIV) and C. gattii (VGI, VGII, VGIII, and VGIV) major molecular types by using the software Saramis Premium (bioMerieux, Marcy-l'Etoile, France). Then, this new database was evaluated for discrimination of the different genotypes. Our in-house database provided correct identification for all C. neoformans and C. gattii genotypes; however, due to the intergenotypic mass spectral similarities, a careful postanalytic evaluation is necessary to provide correct genotype identification.

引用

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