Tapered optical fibres for local pH detection

被引：3

作者：

Martan, T. ^{[1
]}

Pospisilova, M. ^{[1
]}

Aubrecht, J. ^{[1
]}

Mrazek, J. ^{[1
]}

Podrazky, O. ^{[1
]}

Kasik, I. ^{[1
]}

Matejec, V. ^{[1
]}

机构：

[1] AS CR, Inst Photon & Elect, Vvi, Prague 18251, Czech Republic

来源：

2009 INTERNATIONAL WORKSHOP ON INFORMATION OPTICS | 2010年 / 206卷

关键词：

SENSOR;

D O I：

10.1088/1742-6596/206/1/012017

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

This paper deals with preparation of optical probes based on tapered optical fibres and their using for local detection of pH in samples simulating native conditions of plant cells. Optical probes, so-called V-tapers were prepared for this purpose. Fluorescence pH transducer 2',7'-Bis(2-carbonylethyl)-5(6)-carboxyfluorescein was immobilized onto the end-face of cut V-tapers in a thin xerogel layer. Changes of fluorescence-intensity spectra caused by pH changes were employed for pH measurements. Indium-tin oxide or aluminium coated fibre probes with suitable optical and mechanical properties were successfully tested. It has been confirmed that the selected pH transducer is suitable for pH detection from 5.0 to 7.0. This approach has allowed us to determine extra-cellular pH of in-vitro samples.

引用

页数：2

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