Cross-species validation of human specific STR system, SureID® 21G and SureID® 23comp (Health Gene Technologies) in Chimpanzee (Pan Troglodytes)

被引:4
作者
Singh, Abhishek [1 ]
Thakur, Mukesh [1 ]
Sahajpal, Vivek [2 ]
Singh, Sujeet K. [1 ]
Chandra, Kailash [1 ]
Sharma, Arun [2 ]
Devi, Nisha [2 ]
Bernot, Ausma [3 ]
机构
[1] Zool Survey India, Kolkata 700053, W Bengal, India
[2] Directorate Forens Serv, State Forens Sci Lab, Shimla 171218, Himachal Prades, India
[3] Ningbo Hlth Gene Technol Co Ltd, Ningbo 315040, Zhejiang, Peoples R China
关键词
Chimpanzees; Cross reactivity; Human identification; SureID[!sup]®[!/sup] 21G; SureID[!sup]®[!/sup] 23comp;
D O I
10.1186/s13104-019-4801-3
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Objectives: The human specific commercially available STRs system are often not tested in non human primates for their cross applicability. The aim of this study is to test Cross-species validation of two commercially available human specific STR kits i.e. SureID (R) 21G and SureID (R) 23comp (Health Gene Technologies) for their positive application in Chimpanzee (Pan troglodytes). Result: In SureID (R) 21G, 19 loci amplified and while 20 loci amplified in SureID (R) 23comp. All the amplified loci in both STR kits were found polymorphic and the locus Amelogenin showed differential banding patterns between male and female revealing their known gender. The present study validates the applicability of these human specific STR kits in Chimpanzee that can be used in forensics analysis, paternity testing and population genetic studies.
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页数:5
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