Future perspectives in high efficient and ultrafast chiral liquid chromatography through zwitterionic teicoplanin-based 2-μm superficially porous particles

With the aim of pushing forward the limits of high efficient and ultrafast chiral liquid chromatography, a new Chiral Stationary Phase (CSP) has been prepared by covalently bonding the teicoplanin selector on 2.0 mu m Superficially Porous Particles (SPPs). An already validated bonding protocol, which permits to achieve teicoplanin-based CSPs exhibiting zwitterionic behaviour, has been employed to prepare not only the 2.0 mu m version of the CSP but also two other analogous CSPs based, respectively, on 2.7 mu m SPPs and 1.9 mu m Fully Porous Particles (FPPs). The kinetic performance of these CSPs has been compared through the analysis of both van Deemter curves and kinetic plots by employing in-house packed columns of 4.6mm internal diameter and different lengths (20, 50 and 100 mm). In particular on the columns packed with 2.0 mu m SPPs, extremely large efficiencies were observed for both achiral ( >310,000 theoretical plates/meter, Min; h(r): 1.61) and chiral compounds (>290,000 Min; h(r): 1.72) in HILIC conditions. Thanks to their efficiency and enantioselectivity, these CSPs were successfully employed in ultrafast chiral separations. As an example, the enantiomers of haloxyfop were baseline resolved in about 3 s, with a resolution higher than 2.0, (flow rate: 8 mL/min) on a 2 cm long column packed with the 2.0 mu m chiral SPPs. (C) 2017 Elsevier B.V. All rights reserved.