High level of APOBEC3F/3G editing in HIV-2 DNA vif and pol sequences from antiretroviral-naive patients

被引：11

作者：

Bertine, Melanie ^{[1
,2
,3
]}

Charpentier, Charlotte ^{[1
,2
,3
]}

Visseaux, Benoit ^{[1
,2
,3
]}

Storto, Alexandre ^{[3
]}

Collin, Gilles ^{[1
,2
,3
]}

Larrouy, Lucile ^{[1
,2
,3
]}

Damond, Florence ^{[1
,2
,3
]}

Matheron, Sophie ^{[1
,2
,4
]}

Brun-Vezinet, Francoise ^{[3
]}

Descamps, Diane ^{[1
,2
,3
]}

机构：

[1] INSERM, UMR 1137, IAME, Paris, France

[2] Univ Paris Diderot, IAME, UMR 1137, Sorbonne Paris Cite, Paris, France

[3] Hop Bichat Claude Bernard, AP HP, Virol Lab, F-75018 Paris, France

[4] Hop Bichat Claude Bernard, AP HP, Serv Malad Infect & Trop, F-75018 Paris, France

来源：

AIDS | 2015年 / 29卷 / 07期

关键词：

defective viruses; HIV-2; hypermutation; proviral DNA; vif; TO-A MUTATIONS; VIRAL LOAD; ENZYME APOBEC3G; HYPERMUTATION; INFECTION; PROTEIN; DEGRADATION; DEAMINATION; GENOME; COHORT;

D O I：

10.1097/QAD.0000000000000607

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Objective:In HIV-1, hypermutation introduced by APOBEC3F/3G cytidine deaminase activity leads to defective viruses. In-vivo impact of APOBEC3F/3G editing on HIV-2 sequences remains unknown. The objective of this study was to assess the level of APOBEC3F/3G editing in HIV-2-infected antiretroviral-naive patients.Methods:Direct sequencing of vif and pol regions was performed on HIV-2 proviral DNA from antiretroviral-naive patients included in the French Agence Nationale de Recherches sur le SIDA et les hepatites virales CO5 HIV-2 cohort. Hypermutated sequences were identified using Hypermut2.0 program. HIV-1 proviral sequences from Genbank were also assessed.Results:Among 82 antiretroviral-naive HIV-2-infected patients assessed, 15 (28.8%) and five (16.7%) displayed Vif proviral defective sequences in HIV-2 groups A and B, respectively. A lower proportion of defective sequences was observed in protease-reverse transcriptase region. A higher median number of G-to-A mutations was observed in HIV-2 group B than in group A, both in Vif and protease-reverse transcriptase regions (P=0.02 and P=0.006, respectively). Compared with HIV-1 Vif sequences, a higher number of Vif defective sequences was observed in HIV-2 group A (P=0.00001) and group B sequences (P=0.013).Conclusion:We showed for the first time a high level of APOBEC3F/3G editing in HIV-2 sequences from antiretroviral-naive patients. Our study reported a group effect with a significantly higher level of APOBEC3F/3G editing in HIV-2 group B than in group A sequences.

引用

页码：779 / 784

页数：6

共 31 条

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